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Invitrogen
KDEL Polyclonal Antibody, Alexa Fluor™ 555
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KDEL Antibody (PA1-013-A555) in ICC/IF
Immunofluorescent analysis of KDEL/ER (orange) in HeLa cells. The cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 3% Blocker BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a KDEL/ER Rabbit polyclonal antibody, AlexaFluor 555 conjugate (Product # PA1-013-A555), at a concentration of 10 µg/mL in blocking buffer for at least 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst 33342 dye (Product # 62249) at a dilution of 1:10,... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA1-013-A555
应用
建议稀释比
已发表文章
产品规格
种属反应
Hamster,
Human,
Mouse,
Rat
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94
偶联物
Alexa Fluor™ 555
Alexa Fluor™ 555
Alexa Fluor™ 555
激发/发射光谱
553/568 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4, with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
PA1013A555 detects KDEL from human, rat, mouse and hamster.
The PA1013A555 immunogen is a synthetic peptide corresponding to residues C T(643) G E E D T S E K D E L(654) of rat GRP94.
靶标信息
The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ER lumen protein retaining receptor 1; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL (Lys-Asp-Glu-Leu) receptor; KDELR1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; putative MAPK-activating protein PM23; RP23-446N16.1; unnamed protein product
基因别名: 8030486F04Rik; ERD2; ERD2.1; HDEL; I79_020931; PM23
UniProt ID: (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6
Entrez Gene ID: (Human) 10945, (Mouse) 68137, (Rat) 361577, (Chinese hamster) 100750912
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