Invitrogen

KDEL Monoclonal Antibody (2C1), FITC

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KDEL Monoclonal Antibody (2C1), FITC

Product Details

MA5-45870

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:25
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2C1

Immunogen

KDEL peptide (C-AVKDEL -COOH) conjugated at the N-terminal to Bovine Serum Albumin (BSA)
View immunogen

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

Contains

no preservative

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2932324

Product Specific Information

A 1:1,000 dilution of MA5-45870 was sufficient for detection of KDEL in 10 µg of HeLa cell lysates by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects KDEL tagged proteins.

Target Information

The sequence Lys-Asp-Glu-Leu (KDEL) or a closely related sequence, is present at the carboxy-terminus of soluble endoplasmic reticulum (ER) resident proteins and some membrane proteins. 78 and 94 kDa glucose regulated proteins, GRP78 and GRP94 respectively, and protein disulfide isomerase (PDI) all share the C-terminal KDEL sequence. The presence of carboxy-terminal KDEL appears to be necessary for ER retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 78 kDa glucose-regulated protein; 94 kDa glucose-regulated protein; 94 kDa glucose-regulated protein {ECO:0000250|UniProtKB:P08113}; Binding-immunoglobulin protein; BiP; Endoplasmic reticulum chaperone BiP; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Endoplasmic reticulum resident protein 99; Endoplasmin; endoplasmin {ECO:0000250|UniProtKB:P08113}; endothelial cell (HBMEC) glycoprotein; epididymis luminal protein 35; epididymis secretory sperm binding protein Li 125m; epididymis secretory sperm binding protein Li 89n; ER lumen protein retaining receptor 1; ER lumen protein-retaining receptor 1; ERp99; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; gp96 homolog; GRP 78; GRP-78; GRP-94; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); Heat shock protein 70 family protein 5; Heat shock protein 90 kDa beta member 1; heat shock protein 90 kDa beta member 1 {ECO:0000250|UniProtKB:P08113}; heat shock protein 90kDa beta (Grp94), member 1; heat shock protein 90kDa beta family member 1; Heat shock protein family A member 5; Heat shock protein family C member 4; HSP70 family protein 5; hsp90b1 {ECO:0000250|UniProtKB:P08113}; Immunoglobulin heavy chain-binding protein; KDEL; KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; KDEL receptor 1; Lys-Asp-Glu-Leu; Polymorphic tumor rejection antigen 1; Putative MAPK-activating protein PM23; RP23-446N16.1; Steroidogenesis-activator polypeptide; stress-inducible tumor rejection antigen gp96; transforming growth factor alpha regulated gene 2; tumor rejection antigen (gp96) 1; Tumor rejection antigen 1; Tumor rejection antigen gp96; XAP-1 antigen

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Gene Aliases: 8030486F04Rik; AL022860; AU019543; AW215843; baffled; BIP; D2Wsu141e; D2Wsu17e; ECGP; endoplasmin; ERD2; ERD2.1; ERp99; GP96; GRP78; GRP94; HDEL; HEL-S-125m; HEL-S-89n; HEL35; Hsce70; HSP90B1; HSPA5; HSPC4; KDELR1; mBiP; MIF2; PM23; SEZ-7; Sez7; TA-3; Targ2; Tra-1; TRA1

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UniProt ID: (Human) P14625, (Human) P11021, (Mouse) P08113, (Rat) Q66HD0, (Rat) P06761, (Mouse) P20029, (Human) P24390, (Mouse) Q99JH8, (Rat) Q569A6

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Entrez Gene ID: (Human) 7184, (Human) 3309, (Mouse) 22027, (Rat) 362862, (Rat) 25617, (Mouse) 14828, (Human) 10945, (Mouse) 68137, (Rat) 361577

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Function(s)
RNA binding calcium ion binding protein binding ATP binding protein phosphatase binding virion binding low-density lipoprotein particle receptor binding unfolded protein binding glycoprotein binding ATPase activity enzyme binding protein domain specific binding ubiquitin protein ligase binding ribosome binding chaperone binding misfolded protein binding cadherin binding involved in cell-cell adhesion nucleotide binding KDEL sequence binding molecular_function ER retention sequence binding Hsp90 family chaperone membrane trafficking regulatory protein
Process(es)
response to hypoxia toll-like receptor signaling pathway receptor-mediated endocytosis protein transport ER-associated ubiquitin-dependent protein catabolic process retrograde protein transport, ER to cytosol actin rod assembly protein folding in endoplasmic reticulum response to endoplasmic reticulum stress ATF6-mediated unfolded protein response negative regulation of apoptotic process regulation of phosphoprotein phosphatase activity sequestering of calcium ion cellular response to ATP ER overload response activation of signaling protein activity involved in unfolded protein response cerebellum structural organization cerebellar Purkinje cell layer development substantia nigra development positive regulation of cell migration negative regulation of transforming growth factor beta receptor signaling pathway endoplasmic reticulum unfolded protein response positive regulation of protein ubiquitination maintenance of protein localization in endoplasmic reticulum IRE1-mediated unfolded protein response PERK-mediated unfolded protein response cellular response to glucose starvation regulation of protein folding in endoplasmic reticulum cellular response to antibiotic cellular response to manganese ion cellular response to interleukin-4 negative regulation of protein homodimerization activity cell-cell adhesion toxin transport regulation of ATF6-mediated unfolded protein response regulation of IRE1-mediated unfolded protein response regulation of PERK-mediated unfolded protein response positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress protein folding response to stress proteolysis involved in cellular protein catabolic process positive regulation of embryonic development protein retention in ER lumen intracellular protein transport ER to Golgi vesicle-mediated transport retrograde vesicle-mediated transport, Golgi to ER transport biological_process vesicle-mediated transport
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