Invitrogen

KDM5A Recombinant Rabbit Monoclonal Antibody (JB36-60)

Name: KDM5A Recombinant Rabbit Monoclonal Antibody (JB36-60)
Brand: Invitrogen
SKU: MA5-34682

1 篇参考文献

查看其他12个KDM5A抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)

KDM5A Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 5

KDM5A Antibody (MA5-34682) in ICC/IF

Immunofluorescence analysis of KDM5A was performed using 70% confluent log phase SiHa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KDM5A Recombinant Rabbit Monoclonal Antibody (JB36-60) (Product # MA5-34682) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor P... View More

Product Image Gallery

Immunogen image

Immunogen Image KDM5A Recombinant Rabbit Monoclonal Antibody (JB36-60)

产品信息

MA5-34682

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000-1:2,000

查看1篇已发表文章 1篇已发表文章

免疫细胞化学 (ICC/IF)

1:50-1:400

流式细胞分析 (Flow)

1:50-1:100

产品规格

种属反应

Human

已发表种属

Human

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JB36-60

抗原

Recombinant protein within Human KDM5A aa 1,360-1,690

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles, store in dark

运输条件

Ambient (domestic); Wet ice (international)

AB_2848590

产品详细信息

Positive Control: A549, SiHa, SH-SY-5Y.

靶标信息

UPF3B is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The protein is one of two functional homologs to yeast Upf3p. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctionsThis gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. The encoded protein is one of two functional homologs to yeast Upf3p. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein binds to the mRNA and remains bound after nuclear export, acting as a nucleocytoplasmic shuttling protein. It forms with Y14 a complex that binds specifically 20 nt upstream of exon-exon junctions. This gene is located on the long arm of chromosome X. Two splice variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: [histone H3]-trimethyl-L-lysine(4) demethylase 5A; Histone demethylase JARID1A; Jumonji, AT rich interactive domain 1A (RBP2-like); Jumonji/ARID domain-containing protein 1A; lysine (K)-specific demethylase 5A; Lysine-specific demethylase 5A; RBBP-2; retinoblastoma binding protein 2; Retinoblastoma-binding protein 2

查看更多收起

基因别名: JARID1A; KDM5A; RBBP-2; RBBP2; RBP2

查看更多收起

UniProt ID: (Human) P29375

查看更多收起

Entrez Gene ID: (Human) 5927

查看更多收起

功能

DNA binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity protein binding zinc ion binding oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors chromatin DNA binding histone demethylase activity histone modifying enzyme

参与通路

negative regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter spermatogenesis male gonad development circadian regulation of gene expression histone H3-K4 demethylation positive regulation of transcription, DNA-templated oxidation-reduction process negative regulation of histone deacetylase activity