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Invitrogen
KHK Polyclonal Antibody
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FIGURE: 1 / 11
KHK Antibody (PA5-29004) in ICC/IF
Immunofluorescence analysis of KHK was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with KHK Polyclonal Antibody (Product # PA5-29004) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), 1:2000 d... View More
Product Details
PA5-29004
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Pig,
Rat
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human Ketohexokinase Recombinant protein.
Conjugate
Form
Liquid
Concentration
0.4 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol, 1% BSA
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa, HepG2, mouse Liver.
Predicted reactivity: Mouse (87%), Rat (86%), Dog (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
KHK encodes the gene ketohexokinase that catalyzes conversion of fructose to fructose-1-phosphate. The splice variant presented encodes the highly active form found in liver. renal cortex. and small intestine. while the alternate variant encodes the lowe
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: fructokinase; Hepatic fructokinase; Ketohexokinase; ketohexokinase (fructokinase); testicular tissue protein Li 102
Gene Aliases: KETHPRO; KHK
UniProt ID: (Human) P50053, (Mouse) P97328, (Rat) Q02974
Entrez Gene ID: (Pig) 100523241, (Human) 3795, (Mouse) 16548, (Rat) 25659
response to sucrose
response to glucose
response to fructose
response to zinc ion
response to insulin
carbohydrate phosphorylation
fructose catabolic process to hydroxyacetone phosphate and glyceraldehyde-3-phosphate
carbohydrate metabolic process
fructose metabolic process
phosphorylation
regulation of glycogen metabolic process
carbohydrate catabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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