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Rockland
KLH Polyclonal Antibody, AP
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KLH Antibody (600-405-466) in WB
Western Blot of Rabbit anti-KLH (Keyhole Limpet Hemocyanine) antibody. Lane 1: molecular weight. Lane 2: reduced KLH. Load: 1 µg. Primary antibody: KLH antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Goat-anti Rabbit secondary antibody at 1:40,000 for 45 min at RT. Block: 1% BSA-TBS overnight at 4°C. Predicted/Observed size: 350-390 kDa, 350-390 kDa for KLH. Other band(s): none.
产品信息
600-405-466
产品规格
种属反应
Tag
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
KLH (Keyhole Limpet Hemocyanin
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography
保存液
0.05M tris HCl, pH 8, with 0.0001M ZnCl2, 0.001M MgCl2, 50% glycerol, 0.15M NaCl, 10mg/mL BSA
内含物
0.01% sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum and KLH.
靶标信息
Keyhole limpet hemocyanin is an extremely large, heterogeneous glycosylated protein consisting of subunits with a molecular weight of 350,000 and 390,000 in aggregates with molecular weights of 4,500,000-13,000,000. Each domain of a KLH subunit contains two copper atoms that together bind a single oxygen molecule (O2). When oxygen is bound to hemocyanin, the molecule takes on a distinctive transparent, opalescent blue color. The KLH protein is potently immunogenic yet safe in humans and is therefore highly prized as a vaccine carrier protein. The large and highly glycosylated KLH protein cannot be reproduced synthetically. It is available only as a purified biological product from the Keyhole Limpet Megathura crenulata.Keyhole limpet hemocyanin (KLH) is used extensively as a carrier protein in the production of for research, biotechnology and therapeutic applications. Haptens are substances with a low molecular weight such as peptides, small proteins and drug molecules that are generally not immunogenic and require the aid of a carrier protein to stimulate a response from the immune system in the form of antibody production.[2] KLH is the most widely employed carrier proteins for this purpose. KLH is an effective carrier protein for several reasons. Its large size and numerous epitopes generate a substantial immune response, and abundance of lysine residues for coupling haptens, allows a high hapten:carrier protein ratio increasing the likelihood of generating hapten-specific . In addition, because KLH is derived from the limpet, a gastropod, it is phylogenetically distant from mammalian proteins, thus reducing false positives in immunologically based research techniques in mammalian model organisms. KLH may also be a challenging molecule to work with because of its propensity to aggregate and precipitate. Aggregates remain immunogenic, but limit the ability to conjugate haptens and are difficult to manipulate in the laboratory.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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