Invitrogen

KLRG1 Monoclonal Antibody (2F1), Alexa Fluor™ 532, eBioscience™

Name: KLRG1 Monoclonal Antibody (2F1), Alexa Fluor\u2122 532, eBioscience\u2122
Brand: Invitrogen
SKU: 58-5893-82

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12 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)
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KLRG1 Antibody (58-5893-82) in Flow

C57BL/6 mouse splenocytes were stained with NK1.1 Monoclonal Antibody, eFluor 450 (Product # 48-5941-82) and 0.06 µg of Syrian Hamster IgG Isotype Control, Alexa Fluor 532 (Product # 58-4914-81) (left) or 0.06 µg of KLRG1 Monoclonal Antibody, Alexa Fluor 532 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).

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产品信息

58-5893-82

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.125 µg/test

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功能检测 (Functional)

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产品规格

种属反应

Mouse

宿主/亚型

Syrian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2F1

偶联物

Alexa Fluor™ 532 Alexa Fluor™ 532 Alexa Fluor™ 532

激发/发射光谱

534/553 nm 查看光谱

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Ambient (domestic); Wet ice (international)

AB_2815282

产品详细信息

Description: This 2F1 monoclonal antibody reacts with the mouse Killer cell Lectin-like Receptor G1 (KLRG1), also known as Mast cell Function-associated Antigen (MAFA). KLRG1 is a homodimer of glycosylated 30-38 kDa subunits and contains a cytoplasmic motif similar to the immunoreceptor tyrosine-based inhibitory motif (ITIM). Rat MAFA was identified as an antigen specific to rat mast cells; however, the expression of mouse KLRG1/MAFA using 2F1 has not been detected on the surface of mouse mast cell lines, bone marrow-derived mast cells, or peritoneal mast cells. This antigen is expressed on approximately one-third of mouse NK cells and a subset of T cells. MHC class I molecules regulate KLRG1 via interactions with class I-specific inhibitory Ly49 molecules and SHP-1 signaling. Although KLRG1 and Ly49 are both lectin-like inhibitory receptors that are regulated by class I MHC expression, the effects of this on cell surface expression of these molecules are opposing, and the underlying regulatory mechanisms distinct.

Applications Reported: This 2F1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2F1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Alexa Fluor 532 is excited with the Green laser (532 nm) and emits at 561 nm. This cannot be used with the Yellow-Green laser (561 nm). We recommend using a 560/14 band pass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser

靶标信息

KLRG1 (Killer cell lectin-like receptor G1, MAFA, 2F1-Ag) is a homodimeric member of the lectin-like type 2 transmembrane receptor family whose members contain characteristic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) in their cytoplasmic domains. These ITIMs interact with the SH2 domains of protein phosphatases such as SHP-1. KLRG1 is an inhibitory receptor that is expressed on natural killer (NK) cells and certain T cells. NK cells are involved in the lysis of tumor cells and virus-infected cells, and mediate humoral and cell-mediated immune responses.

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