Invitrogen

KTI12 Polyclonal Antibody

查看其他5个KTI12抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (3)

KTI12 Antibody in Immunocytochemistry (ICC/IF)

KTI12 Antibody in Immunocytochemistry (ICC/IF)

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KTI12 Antibody (PA5-98683) in ICC/IF

Immunofluorescent analysis of KTI12 in Hela cells using a KTI12 polyclonal antibody (Product # PA5-98683) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

KTI12 Antibody in Immunocytochemistry (ICC/IF)

KTI12 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

KTI12 Antibody in Western Blot (WB)

Product Image Gallery

KTI12 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of KTI12 in Hela cells using a KTI12 polyclonal antibody (Product # PA5-98683) at a dilution of 1:100. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L). Cells were counter-stained with DAPI.

KTI12 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of KTI12 in paraffin embedded human adrenal gland tissue using a KTI12 polyclonal antibody (Product # PA5-98683) at a dilution of 1:300. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

KTI12 Antibody in Western Blot (WB) — Western Blot analysis of KTI12 using a KTI12 Polyclonal antibody (Product # PA5-98683) at a concentration of 3.2 µg/mL. Positive WB detected in: Hela whole cell lysate, SH-SY5Y whole cell lysate, 293T whole cell lysate, U87 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 39 kDa.

Immunogen image

KTI12 Polyclonal Antibody

Immunogen Image KTI12 Polyclonal Antibody

产品信息

PA5-98683

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:5,000

-

免疫组化（石蜡） (IHC (P))

1:200-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-Dependent

-

产品规格

种属反应

Human, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Protein KTI12 homolog protein (110-204AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.24 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% ProClin 300

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

The function of this protein remains unknown.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: KTI12 homolog, chromatin associated; Protein KTI12 homolog; RP11-91A18.3

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基因别名: KTI12; SBBI81; TOT4

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UniProt ID: (Human) Q96EK9, (Rat) Q5I0L7

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Entrez Gene ID: (Human) 112970, (Rat) 685656

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ATP binding protein binding