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KatG/catalase peroxidase (HPI), cyanobacterial Polyclonal Antibody
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KatG/catalase peroxidase (HPI), cyanobacterial Antibody (AS08-374) in WB
10 µg of total protein from Synechocystis sp. PCC 6,803 WT strain (1) and delta kat G strain (2) was separated on a 8-17% gel and blotted to nitrocellulose membrane. Filters where blocked for 1 h and incubated with anti-Kat G antibody (1:30,000 1 h) followed by incubation with HRP-coupled secondary anti-rabbit antibody (1:3,000 1 h). Signal was detected with standard ECL. There are two bands observed in WT ... View More
产品信息
AS08-374
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant full length KatG protein from Synechocystis sp. PCC 6803 with six His-tag on the terminus.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Synechocystis sp. PCC 6803
靶标信息
KatG encodes a heme-dependent catalase-peroxidase that is primarily a soluble, intracellular enzyme, functioning in the bacterial cytosol where it detoxifies reactive oxygen species and interfaces with redox-active substrates. The protein is a large monomeric hemoprotein (approximately 80 kDa) belonging to the catalase-peroxidase family of peroxidase-like enzymes, built around a peroxidase-type fold that houses a b-type heme cofactor and supports access channels to the heme edge for small-molecule substrates; structural studies also support multiple, relatively weak surface-proximal binding sites for isoniazid near heme access regions. Functionally, KatG exhibits dual enzymatic activities: (i) catalase activity that disproportionates hydrogen peroxide to water and oxygen, helping protect the cell from oxidative stress encountered during host infection, and (ii) peroxidase activity that enables one-electron oxidation reactions and is critical for activation of the tuberculosis prodrug isoniazid, with katG variation being a major genetic basis of clinical isoniazid resistance. This catalytic versatility is linked to cooperation between the heme and a distinctive protein-derived Met-Tyr-Trp covalent adduct cofactor that supports efficient catalatic turnover while maintaining peroxidase-like chemistry.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: catalase-peroxidase; CP; HPI; Hydroperoxidase I; peroxidase/catalase
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