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Invitrogen
Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6)
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Ki-67 Antibody (MA5-14520) in ICC/IF
Immunofluorescence analysis of Ki-67 was performed using 70% confluent log phase HeLa cells serum starved (SS) for 36 hours followed by serum release (SR) for 6 hours. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ki-67 Monoclonal Antibody (SP6) (Product # MA5-14520) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) H... View More
产品信息
MA5-14520
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Bovine,
C. elegans,
Cat,
Chicken,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat,
Rhesus monkey,
Sheep
宿主/亚型
Rabbit
/ IgG
Expression System
proprietary
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SP6
抗原
Synthetic peptide within Human Ki67 aa 1200-1300
偶联物
形式
Liquid
浓度
0.033 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.2, with 1% BSA
内含物
0.1% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.
Recommended positive controls:
IHC (P) - Human tonsil and testis tissue, common marmoset spleen tissue, rat esophagus, small intestine and liver tissue, mouse embryonic skin tissue
IHC (F) - Rat lymph node tissue, transgenic mouse spinal cord tissue
ICC/IF - HAP1 cells, human cardiac stem cells, HEp-2 cells, rat cardiomyocytes
Flow - HAP1 cells
靶标信息
Ki-67 is a nuclear protein that is expressed during various stages in the cell cycle, particularly during late G1, S, G2, and M phases. The protein has a forkhead associated domain (FHA) through which it associates with euchromatin at the perichromosomal layer, the centromeric heterochromatin, and the nucleolus. Ki-67 is shown to have a cell cycle dependent topographical distribution with perinucleolar expression at G1, expression in the nuclear matrix at G2, and expression on the chromosomes during M phase. Ki-67 is commonly used as a proliferation marker because it is not detected in G0 cells, but increases steadily from G1 through mitosis. Ki-67 antibodies are useful in establishing the cell growing fraction in neoplasms. In neoplastic tissues, the prognostic value is comparable to the tritiated thymidine-labelling index. The correlation between low Ki-67 index and histologically low-grade tumors is strong. Ki-67 is routinely used as a neuronal marker of cell cycling and proliferation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: Antigen identified by monoclonal antibody Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog; Antigen KI-67; Antigen KI-67 homolog; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; RP11-380J17.2
基因别名: D630048A14Rik; Ki-67; Ki67; KIA; MIB-; MIB-1; MKI67; PPP1R105
UniProt ID: (Human) P46013, (Mouse) E9PVX6
Entrez Gene ID: (Human) 4288, (Rat) 291234, (Mouse) 17345
