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Agrisera
KrP/Kelch repeat-containing protein/galactose oxidase (At3g07720) Polyclonal Antibody
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KrP/Kelch repeat-containing protein/galactose oxidase (At3g07720) Antibody (AS14-2764) in WB
15 µg of total protein from Arabidopsis thaliana Col-0 root or shoot grown on plates with (+) or without iron (-) extracted and denatured with 2 X LaemmLi buffer (100 m M Tris p H 6.8; 12% glycerol, 0.01% bromophenol blue, 2% β-mercaptoethanol, 4% SDS) at 95°C for 5 min followed by 5 min on ice. Samples were separated on 4-20% Mini-PROTEAN TGX Stain-Free gel and blotted 60 min to nitrocellulose membrane usi... View More
产品信息
AS14-2764
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant KrP derived from Arabidopsis thaliana sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
KrP encodes a nuclear, sequence-specific DNA-binding transcription factor of the C2H2 Krueppel-type zinc-finger family that functions as a gap-class regulator of early Drosophila embryonic patterning. The gene produces alternatively spliced protein isoforms, and the encoded protein contains a C-terminal array of C2H2 zinc-finger DNA-binding motifs together with an N-terminal alanine-rich repression region that is sufficient to confer transcriptional repression activity in heterologous reporter assays. During development, KrP activity helps establish proper anterior-posterior segmentation by regulating downstream target genes, and it is additionally required beyond its segmental role for aspects of tissue differentiation, including contributions to nervous system development and Malpighian tubule morphogenesis, consistent with its broader role as a transcriptional regulator that can act in repression and context-dependent control of gene expression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ICK; KRP
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