Invitrogen

Ku80 Monoclonal Antibody (MEM-54)

Name: Ku80 Monoclonal Antibody (MEM-54)
Brand: Invitrogen
SKU: MA1-10390

高级验证

查看其他40个Ku80抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)
高级验证 (1)

Ku80 Antibody in Immunocytochemistry (ICC/IF)

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Ku80 Antibody (MA1-10390) in ICC/IF

Immunofluorescence analysis of Ku80 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Ku80 Monoclonal Antibody (MEM-54) (Product # MA1-10390) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at... View More

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Ku80 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with Ku80 siRNA and loss of specific signal was observed in immunofluorescence application using Ku80 Monoclonal Antibody (MEM-54) (Product # MA1-10390). {KD}

Immunogen image

Immunogen Image Ku80 Monoclonal Antibody (MEM-54)

产品信息

MA1-10390

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

免疫沉淀 (IP)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

MEM-54

抗原

human whole T-lymphocytes

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1.0 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

15mM sodium azide

保存条件

4°C, do not freeze

运输条件

Ambient (domestic); Wet ice (international)

AB_11154195

产品详细信息

This antibody reacts with Ku80, a 80 kDa subunit of Ku autoantigen (heterodimer of 72 and 87 kDa intracellular polypeptides). Ku autoantigen is involved in DNA repair and in V(D)J recombination through its ability to bind DNA double-strand breaks.

Immunocytochemistry: PFA-fixation possible.

靶标信息

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; FLJ39089; HGNC:12833; Ku autoantigen, 80kDa; Ku-80; Ku-86; Ku80; Ku86; Ku86 autoantigen related protein 1; Lupu; lupus; Lupus Ku autoantigen protein p86; Nuclear factor IV; OTTHUMP00000164061; OTTHUMP00000206791; Thyroid-lupus autoantigen; TLAA; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5

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基因别名: G22P2; KARP-1; KARP1; KU80; Ku86; KUB2; NFIV; XRCC5

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UniProt ID: (Human) Q53HH7

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Entrez Gene ID: (Human) 7520

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功能