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Invitrogen
LC3B Polyclonal Antibody
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LC3B Antibody (PA1-16930) in IHC (P)
Immunohistochemical analysis of LC3B in formalin-fixed paraffin-embedded tissue section of mouse brain. Samples were incubated in LC3B polyclonal antibody (Product # PA1-16930) using a dilution of 1:200 followed by HRP-conjugated secondary antibody with DAB (3, 3 -diaminobenzidine) reagent. Nuclei of cells were counterstained using hematoxylin. This LC3B antibody generated a low to moderate levels of cytopl... View More
Product Details
PA1-16930
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Avian,
Bacteria,
Bovine,
Dog,
C. elegans,
Chicken,
Fruit fly,
Guinea pig,
Hamster,
Human,
Invertebrate,
Mouse,
Non-human primate,
Pig,
Rabbit,
Reptile,
Rat,
Zebrafish
Published species
C. elegans,
Human,
Mouse,
Non-human primate,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide made to an N-terminal portion of the human LC3B protein sequence (between residues 1-100).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The reaction with mouse LC3 is weaker than human.
The immunogen for this antibody production has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins.
The mouse detection has been reported to be weaker than the human. Immunogen sequence homology: Xenopus 84%.
Target Information
LC3B (Autophagy Marker Light Chain 3B, MAP1A/MAP1B LC3 B) in humans, is encoded by the gene MAP1LC3B (Microtubule-associated proteins 1A/1B light chain 3B). LC3B is associated with microtubule assembly and important is in neurogenesis. Recent studies indicate that LC3B plays a vital role in autophagy, a process that involves the bulk degradation of cytoplasmic component. Three human LC3 isoforms undergo post-translational modifications during autophagy. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). LC3B is a microtubule-associated protein that mediate the physical interactions between microtubules and components of the cytoskeleton. LC3B may play a role in processes involving cancer, aging, metabolic and neurodegenerative disorders and cardiovascular/pulmonary diseases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ATG8E; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3; LC3 beta; LC3A; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; MAP1BLC3; MAP1LC3A; microtubule associated protein 1 light chain 3 beta 2; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1 light chain 3 beta 2; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3 beta 2; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B-like; Microtubule-associated proteins 1A/1B light chain 3B-like protein; RP11-346K17.1
Gene Aliases: 1010001C15Rik; Atg8; ATG8F; ATG8G; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1LC3; MAP1LC3B; MAP1LC3B-a; MAP1LC3B2; Mpl3; wu:fb60g11; zbs559; zgc:56434
UniProt ID: (Bovine) O41515, (Human) Q9GZQ8, (Human) A6NCE7, (Rat) Q62625, (Mouse) Q9CQV6
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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