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Invitrogen
LCK Monoclonal Antibody (LCK-01)
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LCK Antibody (MA1-19197) in WB
Western blotting analysis (reducing conditions) of human Lck in whole cell lysate using anti-human Lck (LCK-01) Monoclonal antibody (Product # MA1-19197). Lane 1: J. CaM-1.6 cell line (a mutant derivate of the JURKAT cell line) transfected with Lck; Lane 2: HEK-293T cell line transfected with Lck; Lane 3: HEK-293T cell line (non-transfected).
产品信息
MA1-19197
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
LCK-01
抗原
Peptide corresponding to amino acids 22-36 in the sequence of human Lck.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
15mM sodium azide
保存条件
4°C, do not freeze
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen).
This antibody will not cross-react with mouse.
靶标信息
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56(LSTRA) protein-tyrosine kinase; p56-LCK; Protein YT16; Proto-oncogene Lck; proto-oncogene tyrosine-protein kinase LCK; RP4-675E8.4; T cell-specific protein-tyrosine kinase; T-lymphocyte specific protein tyrosine kinase p56lck; tyr; Tyrosine-protein kinase Lck
基因别名: IMD22; LCK; LSK; p56lck; pp58lck; YT16
UniProt ID: (Human) P06239
Entrez Gene ID: (Human) 3932
glycoprotein binding
protein tyrosine kinase activity
non-membrane spanning protein tyrosine kinase activity
protein serine/threonine phosphatase activity
protein binding
ATP binding
protein C-terminus binding
kinase activity
protein kinase binding
protein phosphatase binding
SH2 domain binding
CD4 receptor binding
CD8 receptor binding
identical protein binding
phosphatidylinositol 3-kinase binding
phosphatidylinositol-4,5-bisphosphate 3-kinase activity
ATPase binding
protein phosphorylation
protein dephosphorylation
cellular zinc ion homeostasis
activation of cysteine-type endopeptidase activity involved in apoptotic process
transmembrane receptor protein tyrosine kinase signaling pathway
regulation of phosphatidylinositol 3-kinase signaling
viral process
hemopoiesis
platelet activation
T cell differentiation
T cell costimulation
peptidyl-tyrosine autophosphorylation
regulation of cell proliferation
response to drug
innate immune response
phosphatidylinositol phosphorylation
phosphatidylinositol-mediated signaling
regulation of defense response to virus by virus
T cell receptor signaling pathway
B cell receptor signaling pathway
positive regulation of T cell receptor signaling pathway
positive regulation of T cell activation
leukocyte migration
release of sequestered calcium ion into cytosol
regulation of lymphocyte activation
positive regulation of intrinsic apoptotic signaling pathway
