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Invitrogen
LCK Monoclonal Antibody (LCK-01), Alexa Fluor™ 647
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LCK Antibody (MA5-18166) in Flow
Separation of human LCK positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human LCK (LCK-01) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18166), concentration in sample 8 µg/mL.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-18166
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
LCK-01
抗原
Peptide corresponding to amino acids 22-36 in the sequence of human Lck
偶联物
Alexa Fluor™ 647
Alexa Fluor™ 647
Alexa Fluor™ 647
激发/发射光谱
650/671 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Size-exclusion chromatography
保存液
PBS
内含物
15mM sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
This antibody recognizes defined epitope (aa 22-36) of Lck, a 56 kDa Src-family protein tyrosine kinase (intracellular antigen). This antibody will not cross-react with mouse.
靶标信息
LCK is a member of the Src-family tyrosine kinase, which are essential for signaling through the T-cell receptor (TCR) complex. Upon TCR triggering, LCK phosphorylates the ITAM motives in its zeta subunits establishing binding sites for the SH2 domains of the tyrosine kinase ZAP70, which is also phosphorylated by LCK and activated to generate subsequent signaling platforms by phosphorylation of adaptor LAT. The majority of LCK is localized to the plasma membrane, however, there is also a significant fraction associated with the Golgi apparatus which may contribute to Raf activation under conditions of weak stimulation through the TCR. LCK contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. LCK is also involved in the regulation of apoptosis induced by various stimuli, but not by the death receptors. Diseases associated with LCK include Immunodeficiency 22 and CD45 deficiency. Alternatively splice variants of the LCK gene encoding different isoforms of the protein have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: EC 2.7.10.2; kinase Lck; Leukocyte C-terminal Src kinase; LSK; Lymphocyte cell-specific protein-tyrosine kinase; OTTHUMP00000008640; OTTHUMP00000008740; OTTHUMP00000008741; p56-LCK; Protein YT16; Proto-oncogene Lck; RP4-675E8.4; T cell-specific protein-tyrosine kinase; tyr; Tyrosine-protein kinase Lck
基因别名: LCK
Entrez Gene ID: (Human) 3932
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