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Agrisera
LEA6-1/Late embryogenesis abundant protein LEA6-1 Polyclonal Antibody
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LEA6-1/Late embryogenesis abundant protein LEA6-1 Antibody (AS22-4836) in WB
Different amounts (25,50,100 and 400 ng) of the purified recombinant At LEA 6-1 protein in LaemmLi buffer 1 X were denatured at 75°C for 5 min, loaded and separated on 15% SDS-PAGE. Gel was blotted 1 h to nitrocellulose membranes (Amersham™ Protam™ Premium 0.45 µM NC) using a tank transfer system (BIO-RAD). Blots were blocked ON with TBS-T/NF-Milk (5%) at 8 °C with agitation. Blot was incubated in the prima... View More
产品信息
AS22-4836
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Recombinant Arabidopsis thaliana LEA6-1.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
LEA6-1 encodes a late embryogenesis abundant (LEA) group 6 protein that accumulates predominantly in seeds and is inducible by water-deficit-related cues; the protein is primarily localized to the cytosol and can also be detected in the nucleus, consistent with the predominantly cytosolic/nucleocytosolic distribution reported for LEA6-family members in Arabidopsis. Structurally, LEA6-1 is a small, highly hydrophilic protein dominated by an intrinsically disordered conformational state in aqueous conditions, with the capacity to gain limited ordered secondary structure (notably helical propensity) under dehydration-mimicking environments; such disorder-to-order behavior is characteristic of LEA proteins and is thought to support reversible interactions with macromolecules and membranes during stress. Functionally, LEA6-1 is implicated in cellular protection during desiccation/osmotic stress by contributing to maintenance of the intracellular "glassy" state and preservation of protein and membrane integrity, thereby supporting seed longevity and enhancing tolerance of early germination/seedling growth to high osmolarity and salinity stress; collectively, these data support a role for LEA6-1 as a stress-responsive, intracellular stabilizer that helps plants withstand low-water availability and related abiotic stresses.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT2G23110; AtLEA6-2.1; AtLEA6-2.1 (At2g23110)
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