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Agrisera
LHY/Late elongated hypocotyl Polyclonal Antibody
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LHY/Late elongated hypocotyl Antibody (AS13-2661) in WB
A clear band for LHY was detected at about 90 k Da after electrophoresis using a 8% polyacrylamide gel cast in a Bio-Rad gel device with 15 wells and 1 mm thickness. Nuclear extract of Arabidopsis thaliana Ws (Wasilewska), zt 0 zt 6 and lhy/cca 1/toc 1 zt 0 was analyzed and protein concentration was equalized according to Bradford quantification to ~10 µg proteins per lane after resuspending and heating to ... View More
产品信息
AS13-2661
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana LHY protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
LHY encodes a MYB-related DNA-binding transcription factor that localizes predominantly to the nucleus, consistent with its role in transcriptional regulation within the plant circadian system. The LHY protein is a single polypeptide containing an N-terminal MYB/SHAQKYF-class DNA-binding domain typical of plant single-repeat MYB factors, enabling sequence-specific promoter binding, and it can participate in higher-order complexes including homo- and heterodimerization with the closely related clock component CCA1. Functionally, LHY is a core element of the Arabidopsis circadian oscillator: its transcript abundance cycles with a morning phase, and perturbation of LHY dosage disrupts circadian rhythms and downstream photoperiodic outputs. Mechanistically, LHY contributes to interlocked transcriptional feedback loops by directly repressing sets of evening-phased clock and output genes via binding to clock-associated cis-elements, thereby shaping daily rhythms in physiology and development (including photoperiodic flowering timing), and genome-wide binding studies further support broader clock-to-physiology coupling such as circadian control of abscisic acid biosynthesis and signaling under stress conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AT1G01060; LATE ELONGATED HYPOCOTYL; LATE ELONGATED HYPOCOTYL 1; LHY1; T25K16.6; T25K16_6
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