Invitrogen

LIMK2 Polyclonal Antibody

Name: LIMK2 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95305

查看其他25个LIMK2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (1)

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LIMK2 Antibody (PA5-95305) in WB

Western blot analysis of LIMK2 in Lane 1: mouse brain tissue lysate (50 µg), Lane 2: mouse liver tissue lysate (50 µg), Lane 3: mouse thymus tissue lysate (50 µg), Lane 4: mouse testis tissue lysate (50 µg), Lane 5: 293T whole cell lysate (40 µg), Lane 6: HeLa whole cell lysate (40 µg). Samples were incubated with LIMK2 polyclonal antibody (Product # PA5-95305).

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产品信息

PA5-95305

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to a sequence at the C-terminus of human LIM kinase 2 (596-635aa KLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRD).

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807108

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: Mouse Brain Tissue, Mouse Liver Tissue, Mouse Thymus Tissue, Mouse Testis Tissue, 293T whole cell, HELA whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

LIMK2 is a serine/threonine kinase involved in regulation of actin cytoskeletal changes via phosphorylation of cofilin. LIMK2 has also been implicated in brain development. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by LIMK2 is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for LIMK2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。