Invitrogen

LRP6 Monoclonal Antibody (A5E7)

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抗体检测数据 (2)

LRP6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LRP6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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LRP6 Antibody (MA5-42555) in IHC (P)

Immunohistochemistry (Paraffin) analysis of paraffin-embedded human colon tissue using LRP6 Monoclonal Antibody (Product # MA5-42555). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the LRP6 antibody at a dilution of 1:100 fo... View More

LRP6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

LRP6 Antibody in Western Blot (WB)

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LRP6 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human colon tissue using LRP6 Monoclonal Antibody (Product # MA5-42555). The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the LRP6 antibody at a dilution of 1:100 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

LRP6 Antibody in Western Blot (WB) — Western blot analysis of LRP6 on Hela cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. LRP6 Monoclonal Antibody (Product # MA5-42555) at 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:20,000 dilution was used for 1 hour at room temperature. Predicted band size: 180 kDa Observed band size: 230 kDa (Glycosylated modification).

产品信息

MA5-42555

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

免疫组化（石蜡） (IHC (P))

1:50-1:200

-

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG2b

分类

Monoclonal

类型

Antibody

克隆号

A5E7

抗原

Recombinant protein within human LRP6 aa 100-300/1613

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

2 mg/mL

纯化类型

Protein G

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2911696

产品详细信息

Positive Control: Hela cell lysates, human colon tissue.

Subcellular Location: Endoplasmic reticulum, Cell membrane, Membrane raft.

靶标信息

LRP6 is essential for the Wnt/beta catenin signaling pathway, probably by acting as a coreceptor together with Frizzled for Wnt. It is a specific, high-affinity receptor for DKK1 and DKK2, but not DKK3. The interaction with DKK1 blocks LRP6-mediated Wnt/beta catenin signaling via LRP6 removal via Kremen proteins-mediated endocytosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: FLJ90062; FLJ90421; low density lipoprotein receptor-related protein 6; Low-density lipoprotein receptor-related protein 6; lpr6; LRP-6

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基因别名: ADCAD2; LRP6; STHAG7

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UniProt ID: (Human) O75581

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Entrez Gene ID: (Human) 4040

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low-density lipoprotein receptor activity receptor binding frizzled binding protein binding Wnt-protein binding kinase inhibitor activity toxin transporter activity apolipoprotein binding identical protein binding protein homodimerization activity Wnt-activated receptor activity coreceptor activity involved in Wnt signaling pathway coreceptor activity involved in canonical Wnt signaling pathway apolipoprotein

参与通路

neural tube closure negative regulation of protein phosphorylation pericardium morphogenesis negative regulation of protein kinase activity positive regulation of cytosolic calcium ion concentration chemical synaptic transmission embryonic pattern specification neural crest formation neural crest cell differentiation Wnt signaling pathway single organismal cell-cell adhesion cerebellum morphogenesis thalamus development cerebral cortex development midbrain-hindbrain boundary development negative regulation of smooth muscle cell apoptotic process external genitalia morphogenesis odontogenesis of dentin-containing tooth response to peptide hormone Wnt signaling pathway involved in dorsal/ventral axis specification canonical Wnt signaling pathway involved in neural crest cell differentiation canonical Wnt signaling pathway involved in regulation of cell proliferation positive regulation of cell cycle positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity palate development convergent extension embryonic retina morphogenesis in camera-type eye canonical Wnt signaling pathway face morphogenesis trachea cartilage morphogenesis cellular response to cholesterol dopaminergic neuron differentiation negative regulation of protein serine/threonine kinase activity protein localization to plasma membrane primitive streak formation negative regulation of canonical Wnt signaling pathway receptor-mediated endocytosis of low-density lipoprotein particle involved in cholesterol transport Wnt signaling pathway involved in somitogenesis axis elongation involved in somitogenesis positive regulation of canonical Wnt signaling pathway toxin transport beta-catenin destruction complex disassembly midbrain dopaminergic neuron differentiation Wnt signaling pathway involved in midbrain dopaminergic neuron differentiation positive regulation of Wnt signaling pathway involved in dorsal/ventral axis specification