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Invitrogen
LSM7 Polyclonal Antibody
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LSM7 Antibody (PA5-143997) in ICC/IF
Immunocytochemistry analysis of LSM7 in MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in LSM7 Polyclonal antibody (Product # PA5-143997) using a dilution of 5 μg/mL. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate fo... View More
产品信息
PA5-143997
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human LSM7 recombinant protein (Position: M1-A103).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.01mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HL-60 whole cell, human HEPG2 whole cell, human U937 whole cell, human HEK293 whole cell, human MCF-7 whole cell, human Jurkat whole cell, human Raji whole cell, rat brain tissue, mouse brain tissue. IHC: human bladder cancer tissue, mouse brain tissue, rat brain tissue, mouse brain tissue, rat brain tissue, rat cerebellum tissue, human melanoma tissue, human ovary cancer tissue, human appendicitis tissue, human bladder cancer tissue, human gastric cancer tissue, human renal carcinoma tissue, human skin cancer tissue, human tonsil tissue, mouse cerebellum tissue. ICC/IF: MCF-7 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
Sm-like proteins were identified in a variety of organisms based on sequence homology with the Sm protein family. Sm-like proteins contain the Sm sequence motif, which consists of 2 regions separated by a linker of variable length that folds as a loop. The Sm-like proteins are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: LSM7 homolog, U6 small nuclear RNA associated; LSM7 U6 small nuclear RNA and mRNA degradation associated; U6 snRNA-associated Sm-like protein LSm7
基因别名: 0910001B06Rik; 1110033F18Rik; LSM7; YNL147W
UniProt ID: (Human) Q9UK45, (Mouse) Q9CQQ8
Entrez Gene ID: (Human) 51690, (Mouse) 66094, (Rat) 362829
