Rockland

Laminin Polyclonal Antibody, Biotin

Name: Laminin Polyclonal Antibody, Biotin
Brand: Rockland
SKU: 600-406-116

查看其他11个Laminin抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Immunogen image

Immunogen Image Laminin Polyclonal Antibody, Biotin

产品信息

600-406-116

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000-1:10,000

免疫组化 (IHC)

1:1,000-1:5,000

酶联免疫吸附实验 (ELISA)

1:20,000-1:100,000

免疫沉淀 (IP)

1:100

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Non-reduced 400 kDa human laminin. Immunization occurred after purity assessment of native protein in non-reducing SDS-PAGE system.

查看免疫原

偶联物

Biotin Biotin Biotin

形式

Lyophilized

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

0.02M potassium phosphate, pH 7.2, with 10mg/mL BSA, 0.15M NaCl

内含物

0.01% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Store vial at 4° C prior to restoration. Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminins and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin antibodies with other human serum proteins or non-Laminin extracellular matrix proteins is negligible.

靶标信息

Laminin is a crucial glycoprotein that plays a significant role in the extracellular matrix, particularly within the basement membrane. It is composed of three subunits: alpha, beta, and gamma chains. These chains are disulfide-linked polypeptides that together form a heterotrimer, resembling the shape of a cross. The human genome encodes 11 genetically distinct laminin chains, which combine to form over 14 different laminin isoforms, each with specific tissue distributions and functions. Laminins can bind to other laminin molecules, as well as integrins and other matrix proteins, providing structural support and influencing cell differentiation, migration, and adhesion. These glycoproteins are vital during early embryonic development, where they contribute to the formation of basement membrane-like structures, even in the absence of type IV collagen. The evolutionary origin of laminin gene families can be traced back to early branching animals, providing insights into metazoan gene family development.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Laminin A chain; Laminin B1 chain; Laminin B2 chain; Laminin M chain; Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit gamma-1; laminin, alpha 1; laminin, alpha 2; laminin, beta 1; laminin, gamma 1 (formerly LAMB2); Laminin-1 subunit alpha; Laminin-1 subunit beta; Laminin-1 subunit gamma; Laminin-10 subunit beta; Laminin-10 subunit gamma; Laminin-11 subunit gamma; Laminin-12 subunit alpha; Laminin-12 subunit beta; Laminin-2 subunit alpha; Laminin-2 subunit beta; Laminin-2 subunit gamma; Laminin-3 subunit alpha; Laminin-3 subunit gamma; Laminin-4 subunit alpha; Laminin-4 subunit gamma; Laminin-6 subunit beta; Laminin-6 subunit gamma; Laminin-7 subunit gamma; Laminin-8 subunit beta; Laminin-8 subunit gamma; Laminin-9 subunit gamma; Merosin heavy chain; S-LAM alpha; S-LAM gamma; S-laminin subunit alpha; S-laminin subunit gamma

查看更多收起

基因别名: CLM; LAMA; LAMA1; LAMA2; LAMB1; LAMB2; LAMC1; LAMM; LIS5; PTBHS; S-LAM-alpha