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Invitrogen
Laminin gamma-2 Recombinant Rabbit Monoclonal Antibody (PSH07-27)
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Laminin gamma-2 Antibody (MA5-60183) in ICC/IF
Immunofluorescence analysis of Laminin gamma-2 (LAMC2) was performed using 70% confluent log phase A-431 and SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Laminin gamma-2 Recombinant Rabbit Monoclonal Antibody (PSH07-27) (Product # MA5-60183... View More
产品信息
MA5-60183
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
PSH07-27
抗原
Recombinant protein within human LAMC2 aa 550-1,193.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Dry ice (international)
RRID
产品详细信息
Positive Control: A431 cell lysate, A549 cell lysate, HaCaT cell lysate, NCI-H441 cell lysate, Mouse skin tissue lysate, Mouse stomach tissue lysate, Rat skin tissue lysate, Rat stomach tissue lysate, human colon tissue, mouse colon tissue, rat colon tissue, A431.
Tissue Specificity: Tissue enhanced (urinary).
Subcellular Location: Secreted, extracellular space, extracellular matrix, basement membrane.
Sequence Similarities: 78% Mouse/Rat.
Predicted band size: 131 kDa.
靶标信息
Laminins, a family of extracellular matrix glycoproteins, are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion, differentiation, migration, signaling, neurite outgrowth and metastasis. Laminins are composed of 3 non identical chains: laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) and they form a cruciform structure consisting of 3 short arms, each formed by a different chain, and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha, beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery, i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown, but some of the chains have been shown to differ with respect to their tissue distribution, presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin, gamma 2. The gamma 2 chain, formerly thought to be a truncated version of beta chain (B2t), is highly homologous to the gamma 1 chain; however, it lacks domain VI, and domains V, IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1, and is specifically localized to epithelial cells in skin, lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin), which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule, and mutations in this gene have been associated with junctional epidermolysis bullosa, a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon, and encoding different isoforms of gamma 2 chain, have been described. The two variants are differentially expressed in embryonic tissues, however, the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: alternatively spliced laminin gamma2 chain; BM600-100kDa; Cell-scattering factor 140 kDa subunit; CSF 140 kDa subunit; Epiligrin subunit gamma; Kalinin subunit gamma; Kalinin/nicein/epiligrin 100 kDa subunit; Ladsin 140 kDa subunit; LAM LAMNB2; lamimin, gamma 2; Laminin B2t chain; laminin gamma2 chain; Laminin subunit gamma-2; laminin, gamma 2; Laminin-5 subunit gamma; Large adhesive scatter factor 140 kDa subunit; Nicein subunit gamma; nicein, 100kD; nicein, 100kDa
基因别名: AA589349; B2T; BM600; CSF; EBR2; EBR2A; LAMB2T; LAMC2; LAMNB2
UniProt ID: (Human) Q13753
Entrez Gene ID: (Human) 3918, (Mouse) 16782, (Rat) 192362
