Search
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Invitrogen
Ly-6C Monoclonal Antibody (HK1.4), Alexa Fluor™ 488, eBioscience™
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
产品信息
53-5932-82
应用
建议稀释比
已发表文章
产品规格
种属反应
Mouse
已发表种属
C. elegans,
Mouse
宿主/亚型
Rat
/ IgG2c, kappa
分类
Monoclonal
类型
Antibody
克隆号
HK1.4
偶联物
激发/发射光谱
499/520 nm
查看光谱
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Affinity chromatography
保存液
PBS, pH 7.2
内含物
0.09% sodium azide
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Description: The monoclonal antibody HK1.4 recognizes mouse Ly-6C, a GPI-linked protein of the Ly6 family. Ly-6C is found on monocytes/macrophages, endothelial cells and granulocytes as well as a subset of lymphocytes. Some variation of expression is found on different mouse strains in regards to expression on CD4 and CD8 lymphocytes. These correlate to 2 alleles both of which are recognized by HK1.4: Ly6c.1 found on C57Bl/6 and SJL cells which results in staining of both CD4 and CD8 cells while Ly6-C.2 found on BALB/c and 3H/He results in staining of CD8, but not CD4 cells.
In vitro addition with HK1.4 antibody can increase proliferation and stimulate cytokine release.
Applications Reported: This HK1.4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HK1.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
生物信息学
蛋白别名: Ly-6C1; Ly6c2; lymphocyte antigen 6 complex, locus C; Lymphocyte antigen 6C1; Lymphocyte antigen Ly-6C
基因别名: AA682074; AA959465; Ly-6C; Ly-6C1; Ly6c; Ly6c1
UniProt ID: (Mouse) P0CW02
Entrez Gene ID: (Mouse) 17067
