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Agrisera
Lysine-tRNA ligase Polyclonal Antibody
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Lysine-tRNA ligase Antibody (AS15-2899) in WB
20 µg of protein from Arabidopsis thylakoids or chloroplasts were separated on 12% SDS-PAGE and blotted 1 h to nitrocellulose membrane using tank transfer. Blots were blocked with 10% Milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:500 overnight with agitation. The antibody solution was decanted and the blot was rinsed briefly twice, then washed three times for 15 min in TBS-T at RT with agitation. Blot was incubated in secondary antibody (anti-rabbit Ig G horseradish pero... View More
产品信息
AS15-2899
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Lysine-tRNA ligase protein sequence of Arabidopsis thaliana.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
Lysine tRNA ligase, also called Lysyl tRNA synthetase, is an essential enzyme that charges tRNA Lys with lysine to enable accurate translation of lysine codons during protein synthesis. It recognizes lysine and tRNA Lys, activates lysine with ATP to form lysyl AMP, and transfers the amino acid to the tRNA, ensuring fidelity via editing and specific identity elements on the tRNA. In plants, lysine tRNA ligase localizes to the cytosol and organelles via distinct isoforms, supporting both cytosolic and organellar translation, and its activity influences growth, stress responses, and development by sustaining protein synthesis capacity. Regulation occurs through expression changes, posttranslational modifications, and interactions with translation factors, and perturbation affects translational efficiency, amino acid homeostasis, and resilience under environmental stress.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: KARS; KARS1; KARS2; KRS; lysine tRNA ligase; LysRS; lysyl tRNA synthetase; Lysyl-tRNA synthetase
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