Invitrogen

M-CSF Monoclonal Antibody (ER-MP58)

Name: M-CSF Monoclonal Antibody (ER-MP58)
Brand: Invitrogen
SKU: MA1-40079

查看其他29个M-CSF抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Immunogen image

Immunogen Image M-CSF Monoclonal Antibody (ER-MP58)

产品信息

MA1-40079

应用

建议稀释比

已发表文章

免疫组化（冰冻） (IHC (F))

1:50

流式细胞分析 (Flow)

1:50

酶联免疫吸附实验 (ELISA)

1:50

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a

分类

Monoclonal

类型

Antibody

克隆号

ER-MP58

抗原

Myeloid precursor cells.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.1 mg/mL

纯化类型

Protein G

保存液

PBS with 0.1% BSA

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2261019

产品详细信息

MA1-40079 detects ER-MP58, an antigen present on all mouse macrophage colony stimulating factor (M-CSF) responsive cells.

MA1-40079 has been successfully used in immunohistochemistry (frozen), ELISA, and flow cytometry applications. Typical starting dilution is 1:50.

MA1-40079 recognizes an antigen present on all mouse macrophage colony-stimulating factor (M-CSF) responsive cells in the bone marrow, including the earliest colony-forming myeloid progenitors, as well as by the majority of other myeloid precursors. The antigen is detected on a broad development range of macrophage precursor cells to the monocytic level, but also on granulocytes. Expression is rapidly lost upon maturation beyond the monocytic stage. The antigen disappears in the course of macrophage differentiation. Furthermore the antigen is clearly different from commonly used myeloid markers as Mac-1, F4/80, and Gr-1.

靶标信息

M-CSF (Macrophage colony-stimulating factor, CSF-1) is a survival factor essential for the proliferation and development of monocytes, macrophages, and osteoclast progenitor cells. M-CSF also induces VEGF (vascular endothelial growth factor) secretion by macrophages, thereby mediating mobilization of endothelial progenitor cells and neovascularization. M-CSF is present as several bioactive isoforms that differ in potency and stability. The full-length protein is synthesized as a membrane-spanning protein that can be expressed on the cell surface or further cleaved and modified in the secretory vesicle. Further, M-CSF is a disulfide-bonded homodimer which is processed into one of two isoforms, a glycoprotein or a proteoglycan that has been modified by the addition of chondroitin sulfate to each subunit. Binding of M-CSF to its receptor, c-Fms (CSF-1R or CD115) induces dimerization of the receptor followed by internalization and degradation of the complex. Functionally, M-CSF is known to stimulate differentiation of hematopoietic stem cells to monocyte-macrophage cell populations in culture. M-CSF acts through the CSF receptor 1. Although human M-CSF shows activity on mouse cells, mouse CSF shows no activity on human cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CSF-1; H-MCSF; M-MCSF; Macrophage colony-stimulating factor 1; MGC31930; osteopetrosis; PG-M-CSF; Proteoglycan macrophage colony-stimulating factor

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基因别名: C87615; Csf1; Csfm; MCSF; op

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UniProt ID: (Mouse) P07141

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Entrez Gene ID: (Mouse) 12977

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功能

cytokine activity macrophage colony-stimulating factor receptor binding protein binding growth factor activity protein homodimerization activity

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