Invitrogen

Nanog Monoclonal Antibody (23D2-3C6)

Name: Nanog Monoclonal Antibody (23D2-3C6)
Brand: Invitrogen
SKU: MA1-017

高级验证

17 篇参考文献

查看其他35个Nanog抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)
高级验证 (1)

Nanog Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 9

Nanog Antibody (MA1-017) in ICC/IF

Immunofluorescent analysis of Nanog (green) in H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides. Cells fixed in 4% paraformaldehyde were permeabilized with 0.1% Triton X-100 for 15 minutes at room temperature. Cells were probed with a Nanog monoclonal antibody (Product # MA1-017) at a dilution of 1:200 overnight at 4°C, washed with PBST, and incubated with a FITC-conjugated secondary antibody at a dilution of 1:100 for 1 hour at room temperature. Nuclei (blue) were stained with DAPI and cells were analyzed by ... View More

Product Image Gallery

Nanog Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Western Blot analysis using Anti-Nanog Monoclonal Antibody (23D2-3C6) (Product # MA1-017) shows increased expression of Nanog in NTERA-2, NCCIT in comparison to HeLa, also the expression is decreased in iPSC upon differentiation to definitive endoderm. {RE}

Immunogen image

Immunogen Image Nanog Monoclonal Antibody (23D2-3C6)

产品信息

MA1-017

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

查看2篇已发表文章 2篇已发表文章

免疫组化 (IHC)

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:50 -1:100

查看10篇已发表文章 10篇已发表文章

流式细胞分析 (Flow)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

其他PubMed (Misc)

查看2篇已发表文章 2篇已发表文章

产品规格

种属反应

Human

已发表种属

C. elegans, Human, Marsupial, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

23D2-3C6

抗原

Full-length human recombinant protein expressed in bacteria

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA, 30% glycerol

内含物

0.05% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2536677

产品详细信息

Western blot analysis of MA1-017 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. MA1-017 shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).

靶标信息

NANOG (Nanog homeobox) is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. NANOG mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human NANOG protein shares 52% overall amino acid identity with the mouse protein, and 85% identity in the homeodomain. Human NANOG maps to gene locus 12p13.31, while the mouse NANOG maps to gene loci 6 F2. Murine embryonic NANOG expression is detected in the inner cell mass of the blastocyst. Research studies have shown that high levels of human NANOG expression in the undifferentiated N-Tera embryonal carcinoma cell line. Further, NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. The role of NANOG in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 (also known as Oct-4), another germline-specific transcription factor, and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. Experiments have demonstrated that iPS cells could be generated using expression plasmids expressing NANOG, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine. When overexpressed, NANOG promotes cells to enter into S phase and proliferation. Diseases associated with dysfunction in the NANOG protein include tetracarcinoma and germ cell/embryonal cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。