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Invitrogen
SSEA3 Monoclonal Antibody (MC-631)
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SSEA3 Antibody (MA1-020) in ICC/IF
Immunofluorescent analysis of SSEA-3 using anti-SSEA-3 monoclonal antibody (Product # MA1-020) shows expression in human teratocarcinoma NCCIT cells (shown in green) but not in negative control HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were probed with a rat monoclonal antibody recognizing SSEA-3 (Product # MA1-020), at a dilution of 1:50 for at least 1 hour at room t... View More
产品信息
MA1-020
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
已发表种属
C. elegans,
Deer,
Human,
Mammal
宿主/亚型
Rat
/ IgM
分类
Monoclonal
类型
Antibody
克隆号
MC-631
抗原
Fischer rat immunized with 4-8 cell stage mouse embryos
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Affinity chromatography - MBP
保存液
potassium phosphate with 0.15M NaCl, 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
SSEA-3 (stage-specific embryonic antigen 3) is a cell surface antigen, expressed along with SSEA-4, TRA-1-60 and TRA-1-81 in embryonic stem cells, embryonal carcinoma cells, and induced pluripotent stem cells (iPS). In human cells, these surface markers are down-regulated during the differentiation process, while SSEA1 is absent in undifferentiated human stem cells and upregulated on the cell surface after retinoic acid mediated differentiation. Conversely, in mouse stem cells, SSEA-1 expression decreases with differentiation while SSEA-3 and SSEA-4 expression increase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
