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Invitrogen
ICAM-1 Monoclonal Antibody (MEM-111)
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FIGURE: 1 / 5
ICAM-1 Antibody (MA1-19123) in WB
Western blotting analysis of human CD54 using mouse monoclonal antibody MEM-111 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-CD54 Monoclonal antibody (Product # MA1-19123) followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88kDa.
Product Details
MA1-19123
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Rat
Published species
Cat,
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
MEM-111
Immunogen
Burkitt's lymphoma cell line Raji.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus). The expression of CD54 is upregulated by activation; it is expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells.
Western Blot: non-reducing conditions. Immunohistochemistry (paraffin sections): positive tissue: thymus, RE cells.
Target Information
ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD54; cell surface glycoprotein P3.58; epididymis secretory sperm binding protein; Human rhinovirus receptor; ICAM-1; ICAM-1 soluble variant; Intercellular adhesion molecule 1; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor; intercellular adhesion molecule-1 precursor; Ly 47; Major group rhinovirus receptor; major group rhinovirus receptor precusor; MALA2; sCD54; soluble CD54; Surface antigen of activated B cells; unnamed protein product
Gene Aliases: BB2; CD54; ICAM; ICAM1; P3.58
UniProt ID: (Human) P05362, (Bovine) Q95132, (Rat) Q00238
Entrez Gene ID: (Human) 3383, (Bovine) 281839, (Rat) 25464
ovarian follicle development
response to hypoxia
response to amphetamine
T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
acute inflammatory response to antigenic stimulus
T cell antigen processing and presentation
positive regulation of cellular extravasation
cell adhesion
leukocyte cell-cell adhesion
cell aging
sensory perception of sound
regulation of cell shape
response to ionizing radiation
response to sulfur dioxide
response to organic cyclic compound
single organismal cell-cell adhesion
membrane to membrane docking
regulation of cell adhesion
positive regulation of actin filament polymerization
cellular response to nutrient levels
response to lipopolysaccharide
cell adhesion mediated by integrin
response to gonadotropin
response to drug
response to amino acid
positive regulation of GTPase activity
adhesion of symbiont to host
positive regulation of nitric oxide biosynthetic process
response to ethanol
positive regulation of vasoconstriction
response to copper ion
receptor-mediated virion attachment to host cell
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
positive regulation of NF-kappaB transcription factor activity
negative regulation of calcium ion transport
establishment of endothelial barrier
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
cellular response to organic substance
cellular response to alkaloid
cellular response to glucose stimulus
cellular response to interleukin-1
cellular response to tumor necrosis factor
cellular response to hypoxia
establishment of Sertoli cell barrier
regulation of ruffle assembly
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of endothelial cell apoptotic process
regulation of leukocyte mediated cytotoxicity
immune effector process
immune response
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
viral entry into host cell
T cell extravasation
cell-cell adhesion
cellular response to oxygen-containing compound
cellular response to beta-amyloid
cellular response to leukemia inhibitory factor
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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