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Invitrogen
beta-2 Microglobulin Monoclonal Antibody (B2M-01)
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FIGURE: 1 / 10
beta-2 Microglobulin Antibody (MA1-19141) in WB
Western blot was performed using Anti-beta-2 Microglobulin Monoclonal Antibody (B2M-01) (Product # MA1-19141) and a 12 kDa band corresponding to beta-2 Microglobulin was observed across all cell lines tested except for SH-SY5Y and NTERA2 cl.D1, which are reported to express low levels of beta-2 Microglobulin. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), THP-1 (Lane 2), U-937 (Lane 3), SH-SY5Y (Lane ... View More
Product Details
MA1-19141
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Horse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
B2M-01
Immunogen
Purified human beta-2 Microglobulin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
It will not cross-react with mouse, rabbit, canine, chicken or bovine.
Western Blot: Both reducing and non-reducing conditions. Non-reducing conditions preferred; RIA: Use at an assay dependent concentration. The dissociation constant of the antibody soluble beta2-microglobulin is 1.5 x 10^-8 mol/L as determined by competitive RIA.
Target Information
Beta-2-microglobulin (B2M) is a 12-14 kDa protein that is a subunit of the major histocompatibility complex (MHC) class I. Human beta 2 Microglobulin associated with HLA Class I antigens are expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells, but is absent on erythrocytes. Beta-2 microglobulin is also found in free form in the plasma. The amino acid sequence of Beta-2-microglobulin is homologous to the constant regions of immunoglobulin heavy and light chains, with a predominantly beta pleated sheet structure. The protein is ubiquitously expressed in the cytoplasm, and is involved in antigen processing and presentation. Beta-2-microglobulin also plays a role in TCR signaling, and downstream signaling in naive CD8+ cells. Beta 2-microglobulin is encoded by the B2M gene located on chromosome 15. A mutation in the Beta-2-microglobulin gene has been shown to result in hypercatabolic hypoproteinemia. Levels of beta 2 microglobulin can be used to assess renal function in kidney transplant recipients. Beta-2-microglobulin that protein can be used as a prognostic marker for HIV infection.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: B-F; B2-microglobulin; B2M; Beta 2 microglobin; Beta 2 microglobulin; beta 2-microglobulin; beta chain of MHC class I molecules; beta-2-microglobin; Beta-2-microglobulin; Beta2 microglobulin; HDCMA22P; MHC Class I; microglobulin beta-2; Orla-B2m; similar to Homo sapiens mRNA for beta 2-microglobulin with GenBank Accession Number AB021288; unnamed protein product
Gene Aliases: AMYLD6; B2M; CDABP0092; HDCMA22P; IMD43; MHC1D4
UniProt ID: (Human) P61769
Entrez Gene ID: (Human) 567
T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
response to molecule of bacterial origin
immune system process
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent
peptide antigen assembly with MHC class I protein complex
peptide antigen assembly with MHC class II protein complex
positive regulation of T cell cytokine production
regulation of membrane depolarization
iron ion transport
cellular iron ion homeostasis
immune response
sensory perception of smell
learning or memory
response to metal ion
negative regulation of neuron projection development
antibacterial humoral response
antigen processing and presentation of endogenous peptide antigen via MHC class I
antigen processing and presentation of exogenous peptide antigen via MHC class II
T cell differentiation in thymus
transferrin transport
regulation of iron ion transport
negative regulation of iron ion transport
protein refolding
innate immune response
regulation of erythrocyte differentiation
positive regulation of receptor-mediated endocytosis
negative regulation of receptor-mediated endocytosis
negative regulation of epithelial cell proliferation
negative regulation of neurogenesis
positive regulation of immune response
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
positive regulation of T cell activation
protein homotetramerization
multicellular organismal iron ion homeostasis
antimicrobial humoral immune response mediated by antimicrobial peptide
cellular response to lipopolysaccharide
cellular response to iron ion
cellular response to iron(III) ion
cellular response to nicotine
amyloid fibril formation
positive regulation of cellular senescence
negative regulation of forebrain neuron differentiation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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