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Invitrogen
Calprotectin Monoclonal Antibody (MAC387)
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FIGURE: 1 / 19
Calprotectin Antibody (MA1-81381) in ICC/IF
Immunofluorescence analysis of Calprotectin (S100A9) was performed using 70% confluent log phase THP-1 and Raji cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Calprotectin (S100A9) Monoclonal Antibody (MAC387) (Product # MA1-80155) at 1:250 dilution ... View More
Product Details
MA1-81381
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Deer,
Horse,
Cat,
Guinea pig,
Goat,
Human,
Non-human primate,
Pig,
Rabbit,
Rat
Published species
Bovine,
Cynomolgus monkey,
Dog,
Horse,
Human,
Mouse,
Pig,
Rat,
Rhesus monkey
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MAC387
Immunogen
Human monocytes.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species.
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase prior to IHC staining. A suggested positive control is human spleen. For flow cytometry, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
The S100A8/A9 heterocomplex, also called MRP-8/MRP-14 complex or calprotectin, comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. The S100A8/A9 complex has antibacterial, antifungal, immunomodulating and antiproliferative effects. Besides this it is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore calprotectin is also detectable in feces. Elevated levels of calprotectin have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AI323541; B8Ag; BEE11; BEE22; calcium binding protein; S100 protein; calgranulin A; calgranulin B; Calgranulin-A; Calgranulin-B; calprotectin; Calprotectin L1H subunit; Calprotectin L1L subunit; CFAG; Cystic fibrosis antigen; intracellular calcium-binding protein (MRP14); Leukocyte L1 complex heavy chain; Leukocyte L1 complex light chain; macrophage migration inhibitory factor-related protein-14; macrophage migration inhibitory factor-related protein-8; Migration inhibitory factor-related protein 14; Migration inhibitory factor-related protein 8; MRP-14; MRP-8; Myeloid-related protein 14; Neutrophil cytosolic 23 kDa protein; Neutrophil cytosolic 7 kDa protein; p14; p23; P7; p8; Protein S100-A8; Protein S100-A9; S100 calcium binding protein A8; S100 calcium binding protein A8 (calgranulin A); S100 calcium binding protein A9 (calgranulin B); S100 calcium-binding protein A8; S100 calcium-binding protein A8 (calgranulin A); S100 calcium-binding protein A9; S100 calcium-binding protein A9 (calgranulin B); S100A8/S100A9; Urinary stone protein band A
Gene Aliases: 60B8AG; CAGA; CAGB; CFAG; CGLA; CGLB; CP-10; L1AG; LIAG; MA387; MAC387; MIF; MRP-14; MRP-8; MRP14; MRP8; NIF; P14; P8; S100A8; S100A9
UniProt ID: (Bovine) P28782, (Human) P05109, (Bovine) P28783, (Rabbit) P50117, (Human) P06702, (Rat) P50115, (Rat) P50116
Entrez Gene ID: (Guinea pig) 100735514, (Horse) 100061766, (Dog) 490461, (Bovine) 616818, (Pig) 100127488, (Rabbit) 100008985, (Goat) 102168876, (Human) 6279, (Dog) 490463, (Bovine) 532569, (Pig) 100127489, (Horse) 100061730, (Rabbit) 100008704, (Goat) 102182273, (Human) 6280, (Rat) 116547, (Guinea pig) 100735238, (Rat) 94195
leukocyte migration involved in inflammatory response
positive regulation of peptide secretion
autophagy
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
astrocyte development
peptidyl-cysteine S-nitrosylation
neutrophil chemotaxis
innate immune response
positive regulation of inflammatory response
neutrophil aggregation
cellular oxidant detoxification
positive regulation of intrinsic apoptotic signaling pathway
cytokine production
acute inflammatory response
chronic inflammatory response
inflammatory response
response to zinc ion
positive regulation of cell growth
sequestering of zinc ion
response to lipopolysaccharide
chemokine production
wound healing
defense response to bacterium
response to ethanol
defense response to fungus
positive regulation of NF-kappaB transcription factor activity
regulation of cytoskeleton organization
regulation of translation
signal transduction
cell-cell signaling
positive regulation of blood coagulation
actin cytoskeleton reorganization
peptidyl-cysteine S-trans-nitrosylation
regulation of integrin biosynthetic process
leukocyte chemotaxis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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