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Invitrogen
ATP Synthase beta Monoclonal Antibody (4.3E8.D10)
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ATP Synthase beta Antibody (MA1-930) in ICC/IF
Immunofluorescent analysis of ATP Synthase beta (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without ATP Synthase beta mouse monoclonal antibody (Product # MA1-930), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with ... View More
产品信息
MA1-930
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
C. elegans,
Human,
Mouse,
Rabbit,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
4.3E8.D10
抗原
Intact rat mitochondria.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
purified
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA1-930 detects the beta subunit of ATP synthase from mouse rat and human samples. This antibody is useful as a mitochondrial marker.
MA1-930 has been successfully used in immunofluorescence, western blot, and immunoprecipitation procedures. By immunoprecipitation, this antibody detects an 50 kDa protein representing ATP synthase from solubilized rat brain mitochondria.
靶标信息
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit
基因别名: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271
UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480
Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947
transporter activity
protein binding
ATP binding
ATPase activity
transmembrane transporter activity
MHC class I protein binding
proton-transporting ATP synthase activity, rotational mechanism
proton-transporting ATPase activity, rotational mechanism
calcium ion binding
lipoprotein particle receptor activity
nucleotide binding
hydrolase activity
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
ATP synthase
angiogenesis
osteoblast differentiation
generation of precursor metabolites and energy
lipid metabolic process
ATP biosynthetic process
negative regulation of cell adhesion involved in substrate-bound cell migration
mitochondrion organization
ATP hydrolysis coupled proton transport
proton transport
mitochondrial ATP synthesis coupled proton transport
regulation of intracellular pH
receptor-mediated endocytosis
ATP synthesis coupled proton transport
ATP metabolic process
transport
ion transport
hydrogen ion transmembrane transport
