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Invitrogen
HSP60 Monoclonal Antibody (4B9/89), Alexa Fluor™ 488
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FIGURE: 1 / 2
HSP60 Antibody (MA3-012-A488) in ICC/IF
Immunofluorescent analysis of Hsp60 (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Hsp60 Monoclonal Antibody, AlexaFluor 488 conjugate (Product # MA3-012-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Imag... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-012-A488
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
4B9/89
Immunogen
Human placental HSP60
Conjugate
Alexa Fluor™ 488
Alexa Fluor™ 488
Alexa Fluor™ 488
Excitation/Emission Max
499/520 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The MA3012A488 antigen is human placental HSP60. Epitope mapping studies using human HSP60 deletion mutants suggest that this antibody binds either between amino acids 335-366 or 484-547.
Target Information
This gene encodes a member of the chaperonin family. The encoded mitochondrial protein may function as a signaling molecule in the innate immune system. This protein is essential for the folding and assembly of newly imported proteins in the mitochondria. This gene is adjacent to a related family member and the region between the 2 genes functions as a bidirectional promoter. Several pseudogenes have been associated with this gene. Two transcript variants encoding the same protein have been identified for this gene. Mutations associated with this gene cause autosomal recessive spastic paraplegia 13.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 60 kDa chaperonin; 60 kDa heat shock protein; 60 kDa heat shock protein, mitochondrial; Chaperonin 60; CPN60; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1; heat shock 60kDa protein 1 (chaperonin); Heat shock protein; Heat shock protein 60; heat shock protein 60 (liver); Heat shock protein family D member 1; heat shock protein, 60 kDa; HSP; HSP-60; HSP-65; Hsp60s1; HuCHA60; Mitochondrial matrix protein P1; P60 lymphocyte protein
Gene Aliases: 60kDa; HSP60; HSPD1; Hspd1-30p
UniProt ID: (Mouse) P63038, (Rat) P63039
Entrez Gene ID: (Mouse) 15510, (Rat) 63868
nucleotide binding
lipopolysaccharide binding
protease binding
p53 binding
double-stranded RNA binding
ATP binding
ubiquitin protein ligase binding
protein complex binding
insulin binding
poly(A) RNA binding
protein heterodimerization activity
chaperone binding
misfolded protein binding
protein binding
detection of misfolded protein
B cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
positive regulation of T cell mediated immune response to tumor cell
protein folding
activation of cysteine-type endopeptidase activity involved in apoptotic process
response to unfolded protein
positive regulation of interferon-alpha production
positive regulation of interferon-gamma production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
protein refolding
B cell proliferation
T cell activation
B cell activation
positive regulation of macrophage activation
positive regulation of apoptotic process
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
isotype switching to IgG isotypes
positive regulation of inflammatory response
protein stabilization
positive regulation of T cell activation
chaperone mediated protein folding requiring cofactor
response to hypoxia
'de novo' protein folding
response to heat
response to organic substance
response to organic cyclic compound
response to lipopolysaccharide
response to ATP
response to cocaine
response to drug
response to hydrogen peroxide
response to estrogen
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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