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Invitrogen
Cytochrome C Monoclonal Antibody (6H2.B4), Alexa Fluor™ 555
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FIGURE: 1 / 4
Cytochrome C Antibody (MA3-38200-A555) in ICC/IF
Knockdown of Cytochrome C was achieved by transfecting HeLa cells with Cytochrome C specific siRNA (Silencer® select Product # S28898, S28897). Immunofluorescence analysis was performed on Untransfected HeLa cells (panel a-d), transfected with non-specific scrambled siRNA (panels e-h) and transfected with Cytochrome C specific siRNA (panel i-l). Cells were fixed, permeabilized, and labelled with Cytochrome ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-38200-A555
Product Specifications
Species Reactivity
Fruit fly,
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
6H2.B4
Immunogen
Rat cytochrome C
Conjugate
Alexa Fluor™ 555
Alexa Fluor™ 555
Alexa Fluor™ 555
Excitation/Emission Max
553/568 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
50 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with native Cytochrome C, but does not recognize denatured cytochrome C. The antibody recognizes a region in the vicinity of amino acid 62 of rat Cytochrome C.
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APOCYTOCHROME C-A; AT1G22840; AT4G10040; ATCYTC-A; CG17903-PA; CG17903-PB; CRF; Cyt-c-p-PA; Cyt-c-p-PB; CYTC-1; CYTC-2; Cytochrome c; cytochrome c DC4; Cytochrome C, expressed in somatic tissues; Cytochrome c, somatic; CYTOCHROME C-1; CYTOCHROME C-2; CYTOCHROME C-A; Cytochrome c-proximal; cytochrome-c-p; Cytochrome-c-proximal; F19G10.20; F19G10_20; hc1; lethal (2) k13905; RH17228p; T5L19.170; T5L19_170
Gene Aliases: CG17903; CYC; CYC2; CYCS; CYCSA; Cyct-c-p; Cyt C; cyt c-p; Cyt-c; Cyt-c-p; Cyt-c2; cyt.c; CytC; CytC-2; Cytc-p; CYTC2; cytochrome c; DC4; dCyt.C-p; DMc01; Dmel\CG17903; Dmel_CG17903; l(2)k13905; pDMc01
UniProt ID: (Mouse) P62897, (Rat) P62898, (Fruit fly) P84029
Entrez Gene ID: (Mouse) 13063, (Rat) 25309, (Fruit fly) 34996, (Rabbit) 100341175, (Rabbit) 100357840
mitochondrial electron transport, ubiquinol to cytochrome c
mitochondrial electron transport, cytochrome c to oxygen
apoptotic DNA fragmentation
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
hydrogen peroxide metabolic process
ATP synthesis coupled electron transport
oxidative phosphorylation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
oxidation-reduction process
response to ischemia
response to oxidative stress
negative regulation of hydrogen peroxide biosynthetic process
glial cell apoptotic process
positive regulation of cellular respiration
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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