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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • p53 Antibodies
          The product (cat # {{productObj.productId}}) that you're looking for is temporarily unavailable. A suggested replacement is cat # {{productObj.replacementProductId}}. Please contact Technical Support for other suitable replacement options.

          Invitrogen

          p53 Antibody Cocktail

          高级验证
          5个已发表图片
          115 篇参考文献
          查看其他174个p53抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite p53 Antibody Cocktail

          • 抗体检测数据 (8)
          • 已发表图片 (5)
          • 高级验证 (4)
          p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.
          p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          Group 53 Created with Sketch.

          图: 1 / 17

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          p53 Antibody (MA5-14067) in IHC (P)

          Immunohistochemistry analysis of p53 showing staining in the nucleus and weak staining in the cytoplasm of paraffin-embedded human breast carcinoma tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-me... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          p53 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Immunoprecipitation (IP)
          p53 Antibody in ChIP Assay (ChIP)
          p53 Antibody in Immunocytochemistry (ICC/IF)
          p53 Antibody in Immunohistochemistry (IHC)
          p53 Antibody in Western Blot, Immunoprecipitation (WB, IP)
          p53 Antibody in Western Blot (WB)
          p53 Antibody in Western Blot (WB)
          p53 Antibody
          p53 Antibody
          p53 Antibody
          p53 Antibody

          产品信息

          MA5-14067

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.5-1.0 µg/mL
          查看33篇已发表文章 33篇已发表文章

          免疫组化 (IHC)

          -
          查看66篇已发表文章 66篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1-2 µg/mL
          查看4篇已发表文章 4篇已发表文章

          免疫细胞化学 (ICC/IF)

          -
          查看4篇已发表文章 4篇已发表文章

          酶联免疫吸附实验 (ELISA)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫沉淀 (IP)

          2 µg/mL
          查看4篇已发表文章 4篇已发表文章

          染色质免疫沉淀 实验 (ChIP)

          1-3 µg x 10^6 cells
          -

          亲和纯化 (AP)

          -
          查看1篇已发表文章 1篇已发表文章

          其他PubMed (Misc)

          -
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Human, Mouse, Rhesus monkey

          已发表种属

          C. elegans, Human, Mouse, Rat

          宿主/亚型

          Mouse / IgG2a/IgG2b

          分类

          Cocktail

          类型

          Antibody

          克隆号

          DO-7, BP53-12

          抗原

          Recombinant human p53 protein

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.125 mg/mL

          纯化类型

          Protein A

          保存液

          PBS, pH 7.4, with 0.2% BSA

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_10981528

          产品详细信息

          MA5-14067 targets p53 in IHC (P), IP, and WB applications and shows reactivity with Human samples.

          The MA5-14067 immunogen is recombinant human p53 protein.

          靶标信息

          The tumor suppressor protein, p53, is a sequence specific transcription factor that is activated by cellular stress. p53 mediates cell cycle arrest or apoptosis in response to DNA damage or starvation for pyrimidine nucleotides. p53 is up-regulated in response to stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest. The structure of p53 comprises an N-terminal transactivation domain, a central DNA-binding domain, an oligomerisation domain, and a C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization. p53 has been characterized to play a role in blocking the proliferative action of damaged cells and act as an anticancer agent. Phosphorylation of Ser392 in p53 has been shown to associate with the formation of human tumors. In addition, p53 has also been linked to the effects of aging and oxidative stress and an increase in p53 has been linked to deficits in LTP (Long Term Potentiation) in learning and memory. p53 is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families such as Li-Fraumeni syndrome.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: Antigen NY-CO-13; Cellular tumor antigen p53; Cys 51 Stop; FLJ92943; HGNC11998; mutant p53; mutant tumor protein 53; OTTMUSP00000006194; P53; p53 cellular tumor antigen; p53 tumor suppressor; Phosphoprotein p53; transformation-related protein 53; tumor protein 53; Tumor suppressor p53; tumor supressor p53; Tumour Protein p53

          查看更多 收起

          基因别名: bbl; BCC7; bfy; bhy; EGK_08142; LFS1; p44; P53; TP53; TRP53

          查看更多 收起

          UniProt ID: (Human) P04637, (Rhesus monkey) P56424, (Mouse) P02340

          查看更多 收起

          Entrez Gene ID: (Human) 7157, (Rhesus monkey) 716170, (Mouse) 22059

          查看更多 收起

          功能
          RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding protease binding p53 binding DNA binding chromatin binding damaged DNA binding double-stranded DNA binding transcription factor activity, sequence-specific DNA binding copper ion binding protein binding ATP binding transcription factor binding zinc ion binding enzyme binding protein kinase binding protein phosphatase binding receptor tyrosine kinase binding ubiquitin protein ligase binding histone acetyltransferase binding identical protein binding sequence-specific DNA binding protein self-association transcription regulatory region DNA binding protein heterodimerization activity protein N-terminus binding chaperone binding protein phosphatase 2A binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription cofactor binding protein C-terminus binding histone deacetylase regulator activity metal ion binding MDM2/MDM4 family protein binding P53-like transcription factor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter DNA strand renaturation base-excision repair nucleotide-excision repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter protein complex assembly cellular response to DNA damage stimulus DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator ER overload response cell cycle arrest Ras protein signal transduction multicellular organism development cell aging protein localization cell proliferation negative regulation of cell proliferation determination of adult lifespan response to X-ray response to gamma radiation positive regulation of gene expression viral process protein sumoylation cell differentiation negative regulation of cell growth DNA damage response, signal transduction by p53 class mediator positive regulation of histone deacetylation chromatin assembly mitotic G1 DNA damage checkpoint positive regulation of protein oligomerization cellular response to UV cellular response to drug cellular response to glucose starvation intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of apoptotic process positive regulation of apoptotic process negative regulation of apoptotic process entrainment of circadian clock by photoperiod proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of neuron apoptotic process negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter response to antibiotic positive regulation of protein export from nucleus regulation of mitochondrial membrane permeability negative regulation of fibroblast proliferation circadian behavior positive regulation of peptidyl-tyrosine phosphorylation negative regulation of helicase activity protein tetramerization negative regulation of telomerase activity positive regulation of thymocyte apoptotic process positive regulation of cell cycle arrest cellular response to hypoxia cellular response to ionizing radiation intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of release of cytochrome c from mitochondria replicative senescence oxidative stress-induced premature senescence intrinsic apoptotic signaling pathway oligodendrocyte apoptotic process positive regulation of execution phase of apoptosis positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway regulation of signal transduction by p53 class mediator regulation of cell cycle G2/M phase transition positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress positive regulation of reactive oxygen species metabolic process positive regulation of intrinsic apoptotic signaling pathway protein import into nucleus, translocation in utero embryonic development somitogenesis release of cytochrome c from mitochondria T cell proliferation involved in immune response B cell lineage commitment T cell lineage commitment positive regulation of leukocyte migration response to ischemia double-strand break repair transcription, DNA-templated regulation of transcription from RNA polymerase II promoter apoptotic process response to oxidative stress cell cycle transforming growth factor beta receptor signaling pathway gastrulation negative regulation of neuroblast proliferation central nervous system development negative regulation of DNA replication rRNA transcription response to UV response to salt stress embryo development ending in birth or egg hatching positive regulation of cardiac muscle cell apoptotic process programmed cell death cerebellum development negative regulation of transforming growth factor beta receptor signaling pathway chromosome breakage T cell differentiation in thymus regulation of tissue remodeling cellular protein localization multicellular organism growth positive regulation of mitochondrial membrane permeability regulation of cell proliferation response to drug mitochondrial DNA repair regulation of neuron apoptotic process positive regulation of cell cycle negative regulation of proteolysis negative regulation of mitotic cell cycle rhythmic process embryonic organ development negative regulation of smooth muscle cell proliferation regulation of catalytic activity chromosome organization neuron apoptotic process regulation of intracellular pH regulation of cell cycle positive regulation of transcription from RNA polymerase II promoter in response to hypoxia intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress regulation of thymocyte apoptotic process necroptotic process cellular response to UV-C mitotic cell cycle arrest regulation of glycolytic process by positive regulation of transcription from RNA polymerase II promoter positive regulation of cell aging negative regulation of macromitophagy regulation of mitochondrial membrane permeability involved in apoptotic process regulation of intrinsic apoptotic signaling pathway by p53 class mediator regulation of hydrogen peroxide-induced cell death negative regulation of glucose catabolic process to lactate via pyruvate intrinsic apoptotic signaling pathway in response to hypoxia regulation of fibroblast apoptotic process negative regulation of DNA biosynthetic process negative regulation of reactive oxygen species metabolic process regulation of cellular senescence
          It has to be done as per old AB suggested Products section.
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