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Invitrogen
E-cadherin Monoclonal Antibody (EP700Y)
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FIGURE: 1 / 6
E-cadherin Antibody (MA5-14458) in ICC/IF
Immunofluorescence analysis of E-cadherin was performed using 90% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with E-cadherin Monoclonal Antibody (EP700Y) (Product # MA5-14458) at 1:100 dilution in 0.1% BSA, incubated at 4 degr... View More
Product Details
MA5-14458
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
EP700Y
Immunogen
A synthetic peptide derived from the fifth cadherin domain of human cadherin
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-14458 targets E-Cadherin in IHC (P) applications and shows reactivity with Human samples.
The MA5-14458 immunogen is a synthetic peptide derived from the fifth cadherin domain of human cadherin.
Target Information
E-Cadherin (epithelial cadherin) is a classical cadherin from the cadherin (alcium dependent adhesion protein) superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. E-cadherin plays a central role in the growth and development of cells by controlling tissue architecture, and maintenance of tissue integrity. In humans, E-cadherin is encoded by the CDH1 gene present on chromosome 16. Studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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