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Invitrogen
CENPA Monoclonal Antibody (H.577.2)
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CENPA Antibody (MA5-14829) in ICC/IF
Immunofluorescence analysis of CENPA was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CENPA Monoclonal Antibody (H.577.2) (Product # MA5-14829) at 1:200 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # ... View More
产品信息
MA5-14829
产品规格
种属反应
Mouse
已发表种属
C. elegans
宿主/亚型
Rabbit
/ IgG
分类
Monoclonal
类型
Antibody
克隆号
H.577.2
抗原
Synthetic peptide corresponding to the amino terminus of mouse CENP-A protein
偶联物
形式
Liquid
浓度
23.4 µg/mL
纯化类型
Affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
<0.02% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other histone proteins, including Histone H3.
靶标信息
Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CENP A; CENP-A; Centromere protein A; Histone H3-like centromeric protein A
基因别名: Cenp-A; Cenpa
UniProt ID: (Mouse) O35216
Entrez Gene ID: (Mouse) 12615
