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Invitrogen
NEFL Monoclonal Antibody (T.400.5)
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FIGURE: 1 / 13
NEFL Antibody (MA5-14981) in ICC/IF
Immunofluorescence analysis of NF-L was performed using 70% confluent log phase HEK-293 and HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. HEK-293 cells were labeled with NF-L Rabbit Monoclonal Antibody (Product # MA5-14981) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluo... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-14981
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
T.400.5
Immunogen
Synthetic peptide surrounding Glu450 of human Neurofilament-L
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
24 µg/mL
Amount
2.4 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
Target Information
Involved in the maintenance of neuronal caliber, neurofilaments are the intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. Like most other intermediate filament proteins (IFPs), the expression of the different neuronal IFPs is both tissue-specific and developmentally regulated. NF-L is the light or low molecular weight microfilament subunit and runs on SDS-PAGE gels at approximately 70 kDa. Neurofilament are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H. NF-H is the heavy or high molecular weight microfilament subunit and runs on SDS-PAGE gels in the range 180-220 kDa, with some variation in different species.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 68 kDa neurofilament protein; FLJ53642; N-FL; NEF3; NEFL; neurofilament; neurofilament 3; Neurofilament light polypeptide; neurofilament protein L; Neurofilament triplet L protein; neurofilament-l; neurofilament-light; NF-L; NF-M; NFM; NLC; OTTHUMP00000196614
Gene Aliases: AI847934; CMT2E; NEFL; NF-L; NF68; NFL
UniProt ID: (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Mouse) 18039, (Rat) 83613
microtubule cytoskeleton organization
anterograde axonal transport
retrograde axonal transport
peripheral nervous system axon regeneration
axonal transport of mitochondrion
regulation of axon diameter
neurofilament bundle assembly
locomotion
negative regulation of neuron apoptotic process
intermediate filament polymerization or depolymerization
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
protein polymerization
neurofilament cytoskeleton organization
response to sodium arsenite
response to toxic substance
response to organic substance
response to organonitrogen compound
spinal cord development
response to peptide hormone
response to corticosterone
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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