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Invitrogen
NQO1 Monoclonal Antibody (A180), DyLight™ 650
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NQO1 Antibody (MA5-16098) in Flow
Flow cytometry analysis of NQO1 in U-87 cells. Samples were incubated with NQO1 monoclonal antibody (Product # MA5-16098) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. An intracellular stain was performed with primary antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-16098
应用
建议稀释比
已发表文章
产品规格
种属反应
Dog,
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
A180
抗原
Full length recombinant NQO1 from human lung.
偶联物
DyLight™ 650
DyLight™ 650
DyLight™ 650
激发/发射光谱
651/673 nm
查看光谱
形式
Liquid
浓度
0.60 mg/mL
规格
60 µg
纯化类型
Protein G
保存液
50mM sodium borate
内含物
0.05% sodium azide
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
NQO1 is a member of the NAD(P)H dehydrogenase (quinone) family and a cytoplasmic 2-electron reductase. This FAD-binding protein forms homodimers and reduces quinones to hydroquinones. NQO1's enzymatic activity prevents the one electron reduction of quinones that results in the production of radical species. Altered expression of the protein has been seen in many tumors and is also associated with Alzheimer's disease (AD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
How to use the Panel Builder
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生物信息学
蛋白别名: Azoreductase; Diaphorase (NADH/NADPH); diaphorase (NADH/NADPH) (cytochrome b-5 reductase); Diaphorase 4; diaphorase 4 (NADH/NADPH); diaphorase-4; dioxin-inducible 1; DT-diaphorase; DTD; Menadione reductase; NAD(P)H dehydrogenase (quinone); NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase, quinone 1; NAD(P)H menadione oxidoreductase 1, dioxin inducible; NAD(P)H-quinone oxidoreductase; NAD(P)H:menadione oxidoreductase; NAD(P)H:menadione oxidoreductase 1; NAD(P)H:Quinone acceptor oxidoreductase type 1; NAD(P)H:quinone oxidoreductase 1; NAD(P)H:quinone oxireductase; NQ01; Phylloquinone reductase; QR1; Quinone reductase 1; unnamed protein product
基因别名: AV001255; DHQU; DIA4; DTD; Nmo-1; Nmo1; NMOR1; NMORI; NQO1; Ox-1; Ox1; QR1
UniProt ID: (Mouse) Q64669, (Rat) P05982
Entrez Gene ID: (Dog) 610935, (Human) 1728, (Mouse) 18104, (Rat) 24314
RNA binding
NAD(P)H dehydrogenase (quinone) activity
cytochrome-b5 reductase activity, acting on NAD(P)H
protein binding
NADPH dehydrogenase (quinone) activity
identical protein binding
NADH dehydrogenase (quinone) activity
superoxide dismutase activity
oxidoreductase activity
poly(A) RNA binding
oxidoreductase
protein polyubiquitination
ubiquinone metabolic process
xenobiotic metabolic process
nitric oxide biosynthetic process
response to oxidative stress
synaptic transmission, cholinergic
response to toxic substance
removal of superoxide radicals
protein catabolic process
response to lipopolysaccharide
cellular response to oxidative stress
negative regulation of protein catabolic process
vitamin E metabolic process
vitamin K metabolic process
innate immune response
cell redox homeostasis
negative regulation of ferroptosis
superoxide metabolic process
negative regulation of catalytic activity
positive regulation of neuron apoptotic process
oxidation-reduction process
aging
response to nutrient
response to organic cyclic compound
response to estradiol
response to ethanol
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