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Invitrogen
B-Raf (V600E) Recombinant Rabbit Monoclonal Antibody (RM8)
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Product Details
MA5-24661
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
RM8
Immunogen
Peptide corresponding to B-Raf V600E mutant.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4, with 50% glycerol, 1% BSA
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
This gene, B-RAF/BRAF, encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 94 kDa B-raf protein; B-Raf proto-oncogene serine/threonine-protein kinase (p94); B-Raf serine/threonine-protein; murine sarcoma viral (v-raf) oncogene homolog B1; p94; Proto-oncogene B-Raf; serine/threonine protein kinase; Serine/threonine-protein kinase B-raf; v-raf murine sarcoma viral oncogene homolog B; v-Raf murine sarcoma viral oncogene homolog B1
Gene Aliases: B-raf; B-RAF1; BRAF; BRAF-1; BRAF1; NS7; RAFB1
UniProt ID: (Human) P15056
Entrez Gene ID: (Human) 673
nucleotide binding
catalytic activity
protein kinase activity
protein serine/threonine kinase activity
MAP kinase kinase activity
MAP kinase kinase kinase activity
calcium ion binding
protein binding
ATP binding
zinc ion binding
kinase activity
transferase activity
small GTPase binding
identical protein binding
metal ion binding
scaffold protein binding
protein serine kinase activity
MAPK cascade
myeloid progenitor cell differentiation
protein phosphorylation
signal transduction
epidermal growth factor receptor signaling pathway
visual learning
organ morphogenesis
positive regulation of gene expression
negative regulation of fibroblast migration
positive regulation of glucose transport
synaptic vesicle exocytosis
cell differentiation
thyroid gland development
T cell differentiation in thymus
positive regulation of peptidyl-serine phosphorylation
substrate adhesion-dependent cell spreading
somatic stem cell population maintenance
regulation of cell proliferation
negative regulation of apoptotic process
stress fiber assembly
CD4-positive, alpha-beta T cell differentiation
positive T cell selection
CD4-positive or CD8-positive, alpha-beta T cell lineage commitment
negative regulation of neuron apoptotic process
regulation of T cell differentiation
alpha-beta T cell differentiation
thymus development
regulation of axon regeneration
positive regulation of axon regeneration
positive regulation of axonogenesis
T cell receptor signaling pathway
positive regulation of stress fiber assembly
long-term synaptic potentiation
head morphogenesis
face development
ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
cellular response to calcium ion
cellular response to xenobiotic stimulus
endothelial cell apoptotic process
establishment of protein localization to membrane
postsynaptic modulation of chemical synaptic transmission
positive regulation of substrate adhesion-dependent cell spreading
negative regulation of synaptic vesicle exocytosis
negative regulation of endothelial cell apoptotic process
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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