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Invitrogen
Metallothionein Monoclonal Antibody (2B5)
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Metallothionein Antibody (MA5-27586) in WB
Western blot analysis of Metallothionein in pseudomonas aeruginosa metallothionein (pmta) gst tagged using 1 µg of protein. Sample was prepared with 5% milk and TBST, incubated with Metallothionein monoclonal antibody (Product # MA5-27586) using a dilution of 1:1000 (2 hours at RT) and followed by a Goat Anti-Mouse HRP secondary antibody at a dilution of 1:5000 (1 hour at RT). Samples are arranged as follows: Lane 1: molecular weight ladder, Lane 2: GST, Lane 3: Purified Pseudomonas aeruginosa Metallothionein (PmtA) GST tagged.
Product Details
MA5-27586
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bacteria
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2B5
Immunogen
Full length Pseudomonas aeruginosa Metallothionein protein
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A 1:1,000 dilution of MA5-27586 was sufficient for detection of Metallothionein in 1 µg of purified Pseudomonas aeruginosa Metallothionein (PmtA)-GST tagged protein by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects 36 kDa band corresponding to Pseudomonas aeruginosa Metallothionein.
Target Information
Metallothioneins (MTs) are a family of low molecular weight, heavy metal-binding proteins characterized by a high cysteine content and lack of aromatic amino acids. MTs bind 7 to 12 heavy metal atoms per molecule of protein. They are ubiquitous in the animal and plant kingdoms and are also found in prokaryotes. In mammals, the cysteine residues are absolutely conserved and serve to coordinate heavy metal atoms such as zinc, cadmium, and copper via mercaptide linkages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CES 1; CES1; Metallothionein; Metallothionein 2; Metallothionein 2A; Metallothionein IA; Metallothionein II; Metallothionein2; MGC32848; MT 1A; MT 2; MT 2A; MT IA; MT II; MT2; MT2A; Probable pmtA
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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