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Invitrogen
VGLUT3 Monoclonal Antibody (N34/34)
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FIGURE: 1 / 1
VGLUT3 Antibody (MA5-27594) in ICC/IF
Immunofluorescent analysis of VGLUT3 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with VGLUT3 monoclonal antibody (Product # MA5-27594) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) VGlut3 Antibody, D) Merged image. Magnificat... View More
Product Details
MA5-27594
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
N34/34
Immunogen
Fusion protein amino acids 546-588 (cytoplasmic C-terminus) of rat VGLUT3
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27594 was sufficient for detection of VGLUT3 in 20 µg of CV-1 fibroblast cells (lysate) transfected with VGLUT3 by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 65kDa.
This antibody was formerly sold as clone S34-34.
Target Information
Glucose is fundamental to the metabolism of mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-3 is detectable in a few normal cell type spermatids in testis with active spermatogenesis, placental trophoblast membranes, and neurons in brain. GLUT-3 staining is also detectable in human cancers including those of the ovary, lung, and testis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 8; solute carrier family 17 (vesicular glutamate transporter), member 8; Solute carrier family 17 member 8; unnamed protein product; Vesicular glutamate transporter 3; vesicular glutamate transporter-3; VGLUT 3; VGluT3
Gene Aliases: BC042593; DFNA25; SLC17A8; VGLUT3
UniProt ID: (Human) Q8NDX2, (Mouse) Q8BFU8, (Rat) Q7TSF2
Entrez Gene ID: (Human) 246213, (Mouse) 216227, (Rat) 266767
chloride channel activity
L-glutamate transmembrane transporter activity
neurotransmitter transporter activity
sodium:phosphate symporter activity
protein binding
secondary active transmembrane transporter activity
symporter activity
transmembrane transporter activity
L-glutamate uniporter activity
secondary carrier transporter
neural retina development
ion transport
sodium ion transport
phosphate ion transport
chloride transport
neurotransmitter transport
sensory perception of sound
L-glutamate transport
ion transmembrane transport
synaptic transmission, glutamatergic
sodium ion transmembrane transport
sodium-dependent phosphate transport
regulation of synapse structure or activity
regulation of acetylcholine uptake
L-glutamate import
positive regulation of glutamate uptake involved in transmission of nerve impulse
phosphate ion homeostasis
transmembrane transport
cochlea development
neurotransmitter loading into synaptic vesicle
chloride transmembrane transport
transport
L-glutamate transmembrane transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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