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Invitrogen
KCC2 Monoclonal Antibody (N1/12)
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FIGURE: 1 / 6
KCC2 Antibody (MA5-27610) in ICC/IF
Immunofluorescent analysis of KCC2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with KCC2 monoclonal antibody (Product # MA5-27610) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:200, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) KCC2 Antibody, D) Merged image. Magnification: 6... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-27610
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N1/12
Immunogen
Fusion protein amino acids 932-1043 corresponding to rat KCC2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27610 was sufficient for detection of KCC2 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 140kDa.
This antibody was formerly sold as clone S1-12
Target Information
Mediates electroneutral potassium-chloride cotransport in mature neurons. Transport occurs under isotonic conditions, but is activated 20-fold by cell swelling. Important for Cl(-) homeostasis in neurons.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Electroneutral potassium-chloride cotransporter 2; Furosemide-sensitive K-Cl cotransporter; hKCC2; K-Cl cotransporter 2; KCC 2; KCC2a variant 1; KCC2a-S25 variant 1; KCC2b variant 2; mKCC2; Neuronal K-Cl cotransporter; neuronal-specific K-Cl cotransporter; rKCC2; S12A5; Solute carrier family 12 member 5; solute carrier family 12, member 5
Gene Aliases: KCC2; KIAA1176; mKIAA1176; SLC12A5
UniProt ID: (Mouse) Q80TI5, (Rat) Q63633
Entrez Gene ID: (Mouse) 57138, (Rat) 171373
transport
ion transport
potassium ion transport
chloride transport
chemical synaptic transmission
learning
organic cation transport
ammonium transport
multicellular organism growth
thermosensory behavior
response to drug
intracellular pH reduction
transmembrane transport
ammonium transmembrane transport
cation transmembrane transport
chloride transmembrane transport
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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