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Invitrogen
MFN2 Monoclonal Antibody (N153/5)
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FIGURE: 1 / 2
MFN2 Antibody (MA5-27647) in ICC/IF
Immunofluorescent analysis of MFN2 in human neuroblastoma cell line (SK-N-BE). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with MFN2 monoclonal antibody (Product # MA5-27647) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488, Phalloidin Texas Red and DAPI secondary antibody at a dilution of 1:100, 1:1000 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain, B) Phalloidin Texas Red F-Actin stain, C) Mitofusin 2 Antibody, D) Merged image. Magnifica... View More
Product Details
MA5-27647
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Rat
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
N153/5
Immunogen
Fusion protein amino acids 370-600 (cytoplasmic N-terminus) of mouse Mitofusin-2. Rat: 97% identity (226/231 amino acids identical). Human: 92% identity (214/231 amino acids identical) ~55% identity with Mitofusin-1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-27647 was sufficient for detection of Mitofusin-2 in 20 µg of mouse cardiac mitochondrial lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 90kDa. No cross-reactivity against Mitofusin-1.
This antibody was formerly sold as S153-5.
Target Information
This gene encodes a mitochondrial membrane protein that participates in mitochondrial fusion and contributes to the maintenance and operation of the mitochondrial network. This protein is involved in the regulation of vascular smooth muscle cell proliferation, and it may play a role in the pathophysiology of obesity. Mutations in this gene cause Charcot-Marie-Tooth disease type 2A2, and hereditary motor and sensory neuropathy VI, which are both disorders of the peripheral nervous system. Defects in this gene have also been associated with early-onset stroke. Two transcript variants encoding the same protein have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CPRP 1; HSG protein; hyperplasia suppressor; hypertension related protein 1; hypertension-related protein; Hypertension-related protein 1; MARF; GTP-binding transmembrane protein; MFN 2; Mitochondrial assembly regulatory factor; mitochondrial transmembrane GTPase FZO1A; Mitofusin-2; Mitofusin2; Transmembrane GTPase MFN2; unnamed protein product
Gene Aliases: CMT2A; CMT2A2; CMT2A2A; CMT2A2B; CPRP1; D630023P19Rik; Fzo; HMSN6A; HSG; KIAA0214; MARF; MFN2; MSL
UniProt ID: (Human) O95140, (Mouse) Q80U63
Entrez Gene ID: (Human) 9927, (Mouse) 170731, (Rat) 64476
blastocyst formation
protein targeting to mitochondrion
autophagy
apoptotic process
response to unfolded protein
mitochondrion organization
mitochondrial membrane organization
mitochondrial fusion
aerobic respiration
protein localization to pre-autophagosomal structure
negative regulation of Ras protein signal transduction
camera-type eye morphogenesis
negative regulation of smooth muscle cell proliferation
mitochondrion localization
parkin-mediated mitophagy in response to mitochondrial depolarization
positive regulation of cold-induced thermogenesis
positive regulation of vascular smooth muscle cell proliferation
positive regulation of vascular associated smooth muscle cell apoptotic process
multicellular organism development
cell cycle arrest
metabolic process
negative regulation of cell proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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