Invitrogen

PDK1 Recombinant Rabbit Monoclonal Antibody (JA67-30)

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产品信息

MA5-32702

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:5000
-

免疫组化（石蜡） (IHC (P))

1:2,000
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免疫细胞化学 (ICC/IF)

1:50-1:200
-

流式细胞分析 (Flow)

1:50-1:100
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

JA67-30

抗原

Synthetic peptide within Human PDK1 aa 16-65

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

TBS, pH 7.4, with 40% Glycerol, 0.05% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2809979

产品详细信息

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

PDK1 (pyruvate dehydrogenase kinase, isozyme 1) gene, located on chromosome 2q31.1, encodes a member of the pyruvate dehydrogenase kinase family. This kinase plays a crucial role in the regulation of glucose metabolism by phosphorylating and inactivating the pyruvate dehydrogenase complex (PDC), which converts pyruvate to acetyl-CoA, linking glycolysis to the Krebs cycle. Structurally, the PDK1 protein contains a kinase domain that is essential for its enzymatic activity. PDK1 acts as a gatekeeper of glucose oxidation and is tightly regulated by various metabolic signals, including insulin and hypoxia. Dysregulation of PDK1 activity has been implicated in metabolic disorders such as diabetes and cancer, where it contributes to altered cellular energy homeostasis and metabolic reprogramming.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: 3-phosphoinositide dependent protein kinase-1; 3-phosphoinositide-dependent protein kinase 1; 3-phosphoinositide-dependent protein kinase 2 pseudogene; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial; [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial; hPDK1; isoenzyme 1; kinase isoenzyme 1; lipoamide; PDH kinase 1; PDK p48; PkB kinase like gene 1; pyruvate dehydrogenase; pyruvate dehydrogenase kinase 1; Pyruvate dehydrogenase kinase isoform 1; pyruvate dehydrogenase kinase, isoenzyme 1; pyruvate dehydrogenase kinase, isozyme 1

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基因别名: B830012B01; D530020C15Rik; PDK1; PDPK1; PDPK2; PDPK2P; PRO0461

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UniProt ID: (Human) O15530, (Mouse) Q8BFP9

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Entrez Gene ID: (Human) 5170, (Rat) 116551, (Mouse) 228026

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功能
protein serine/threonine kinase activity 3-phosphoinositide-dependent protein kinase activity insulin receptor binding protein binding ATP binding phospholipase activator activity kinase activity protein kinase binding phospholipase binding protein kinase activity pyruvate dehydrogenase (acetyl-transferring) kinase activity protein complex binding protein homodimerization activity protein heterodimerization activity nucleotide binding transferase activity non-receptor serine/threonine protein kinase
参与通路
stimulatory C-type lectin receptor signaling pathway type B pancreatic cell development transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation negative regulation of protein kinase activity hyperosmotic response epidermal growth factor receptor signaling pathway positive regulation of phospholipase activity regulation of endothelial cell migration negative regulation of cardiac muscle cell apoptotic process cell migration peptidyl-serine phosphorylation peptidyl-threonine phosphorylation calcium-mediated signaling actin cytoskeleton organization platelet activation negative regulation of transforming growth factor beta receptor signaling pathway T cell costimulation activation of protein kinase B activity cellular response to insulin stimulus negative regulation of toll-like receptor signaling pathway intracellular signal transduction Fc-epsilon receptor signaling pathway regulation of I-kappaB kinase/NF-kappaB signaling regulation of mast cell degranulation protein autophosphorylation phosphatidylinositol-mediated signaling focal adhesion assembly T cell receptor signaling pathway positive regulation of release of sequestered calcium ion into cytosol cellular response to epidermal growth factor stimulus positive regulation of establishment of protein localization to plasma membrane extrinsic apoptotic signaling pathway glucose metabolic process cell surface receptor signaling pathway cell proliferation intrinsic apoptotic signaling pathway in response to oxidative stress regulation of acetyl-CoA biosynthetic process from pyruvate regulation of glucose metabolic process hypoxia-inducible factor-1alpha signaling pathway carbohydrate metabolic process phosphorylation mitophagy in response to mitochondrial depolarization
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