Invitrogen

Phospho-PI3K p85/p55 (Tyr458, Tyr199) Recombinant Rabbit Monoclonal Antibody (PI3KY458-1A11)

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Datasheet
Protocols
Questions & Answers
Tech Support
Datasheet
Protocols
Questions & Answers
Tech Support
Phospho-PI3K p85/p55 (Tyr458, Tyr199) Recombinant Rabbit Monoclonal Antibody (PI3KY458-1A11)

Product Details

MA5-36953

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

1-1,000 ng/mL
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

PI3KY458-1A11

Immunogen

A synthetic phospho-peptide corresponding to residues surrounding Tyr458 of human phospho PI3K p85.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4, with 50% glycerol, 0.1% BSA

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2896888

Product Specific Information

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

Phosphoinositide 3-kinase (PI3K, phospho PI3K) Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Phospho PI3K is necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. During insulin stimulation, it also binds to IRS-1. Growth factors and hormones trigger this phosphorylation event, which in turn coordinates cell growth, cell cycle entry, cell migration, and cell survival.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: OTTHUMP00000009783; OTTHUMP00000009784; p55PIK; Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase regulatory subunit beta; Phosphatidylinositol 3-kinase regulatory subunit gamma; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85 alpha); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 3 (p55, gamma); phosphatidylinositol 3-kinase-associated p-85 alpha; phosphoinositide-3-kinase regulatory subunit; phosphoinositide-3-kinase regulatory subunit alpha; phosphoinositide-3-kinase regulatory subunit beta; phosphoinositide-3-kinase, regulatory subunit 1 (alpha); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 3 (gamma); PI3-kinase regulatory subunit alpha; PI3-kinase regulatory subunit beta; PI3-kinase regulatory subunit gamma; PI3-kinase subunit p55-gamma; PI3-kinase subunit p85-alpha; PI3-kinase subunit p85-beta; PI3K regulatory subunit alpha; PI3K regulatory subunit beta; PI3K regulatory subunit gamma; polypeptide 3; ptdIns-3-kinase regulatory subunit alpha; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit gamma; ptdIns-3-kinase regulatory subunit p55-gamma; PtdIns-3-kinase regulatory subunit p85-al; ptdIns-3-kinase regulatory subunit p85-beta; RP4-533D7.2

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Gene Aliases: AA414954; AGM7; GRB1; IMD36; MPPH; MPPH1; p50alpha; p55; p55-GAMMA; p55alpha; p55PIK; p85; p85-ALPHA; p85-BETA; p85alpha; P85B; p85beta; PI3K; PIK3R1; PIK3R2; PIK3R3

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UniProt ID: (Human) P27986, (Human) O00459, (Human) Q92569, (Mouse) P26450, (Mouse) O08908, (Mouse) Q64143

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Entrez Gene ID: (Human) 5295, (Human) 5296, (Human) 8503, (Mouse) 18708, (Mouse) 18709, (Mouse) 18710

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Function(s)
transmembrane receptor protein tyrosine kinase adaptor activity insulin receptor binding insulin-like growth factor receptor binding neurotrophin TRKA receptor binding protein binding transcription factor binding 1-phosphatidylinositol-3-kinase activity protein phosphatase binding phosphatidylinositol 3-kinase regulator activity phosphatidylinositol 3-kinase regulatory subunit binding ErbB-3 class receptor binding phosphatidylinositol 3-kinase binding insulin binding insulin receptor substrate binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity 1-phosphatidylinositol-3-kinase regulator activity protein heterodimerization activity GTPase activator activity receptor tyrosine kinase binding receptor binding platelet-derived growth factor receptor binding calmodulin binding protein C-terminus binding protein kinase binding protein domain specific binding estrogen receptor binding ubiquitin protein ligase binding ATPase binding phosphoprotein binding kinase modulator
Process(es)
cellular glucose homeostasis negative regulation of cell-matrix adhesion protein phosphorylation phosphatidylinositol biosynthetic process epidermal growth factor receptor signaling pathway insulin receptor signaling pathway extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage phosphatidylinositol 3-kinase signaling regulation of phosphatidylinositol 3-kinase signaling viral process platelet activation B cell differentiation positive regulation of cell migration T cell costimulation positive regulation of tumor necrosis factor production cellular response to insulin stimulus positive regulation of RNA splicing cellular response to UV response to endoplasmic reticulum stress phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway positive regulation of transcription factor import into nucleus negative regulation of apoptotic process regulation of phosphatidylinositol 3-kinase activity negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of glucose import phosphatidylinositol phosphorylation insulin-like growth factor receptor signaling pathway vascular endothelial growth factor receptor signaling pathway phosphatidylinositol-mediated signaling protein stabilization T cell receptor signaling pathway leukocyte migration regulation of stress fiber assembly NFAT protein import into nucleus growth hormone receptor signaling pathway positive regulation of establishment of protein localization to plasma membrane positive regulation of endoplasmic reticulum unfolded protein response positive regulation of glucose import in response to insulin stimulus regulation of autophagy protein transport positive regulation of GTPase activity regulation of small GTPase mediated signal transduction positive regulation of protein phosphorylation glucose metabolic process transport negative regulation of cell adhesion signal transduction negative regulation of heart rate positive regulation of gene expression negative regulation of muscle cell apoptotic process negative regulation of cell-cell adhesion response to insulin positive regulation of myoblast differentiation negative regulation of blood pressure negative regulation of proteolysis negative regulation of smooth muscle cell proliferation response to glucocorticoid response to cAMP regulation of establishment of protein localization to plasma membrane regulation of actin filament polymerization regulation of phosphorylation positive regulation of cell adhesion
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