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HLA-ABC Monoclonal Antibody (W6/32), FITC
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FIGURE: 1 / 2
HLA-ABC Antibody (MA5-44095) in Flow
Flow Cytometry analysis of HLA-ABC using HLA-ABC Monoclonal Antibody (W6/32), FITC (Product # MA5-44095). Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human HLA Class I (W6/32) FITC antibody (concentration in sample 3 μg/mL).
Product Details
MA5-44095
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Cat,
Human,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
W6/32
Immunogen
Membrane of human tonsil cells
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Size-exclusion chromatography
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody The antibody W6/32 recognizes an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analyzing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
Target Information
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumor defense. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
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Bioinformatics
Protein Aliases: allele B-3515; B*3509; cell surface glycoprotein; integral membrane protein; exons 2-8; glycosylation aa 86, alpha domain 1 aa 1-24, alpha domain 2 aa 25-114, alpha domain 3 aa 207-298; glycosylation aa 86, alpha domain 1 aa 25-114, alpha domain 2 aa 115-206, alpha domain 3 aa 207-298; H-2K; H-2K(d); H2-K; heavy chain; hla antigen; HLA B-1519; HLA class I antigen HLA-B; HLA class I histocompatibility antigen, A alpha chain; HLA class I histocompatibility antigen, A-1 alpha chain; HLA class I histocompatibility antigen, B alpha chain; HLA class I histocompatibility antigen, C alpha chain; HLA protein allele A25; HLA protein allele B37; HLA protein allele B51; HLA protein allele B57; HLA protein allele B7; HLA-A; HLA-A 2402; HLA-A*0201 alpha chain; human leukocyte antigen; HLA-A*0204; HLA-A*0207 alpha chain; human leukocyte antigen; HLA-A-0102 allele; HLA-A24AK; HLA-A25; HLA-B; HLA-B 1502; HLA-B*4002; HLA-B-07ZEL allele; HLA-B-1501; HLA-B-1516; HLA-B-1520 allele; HLA-B-15AOH allele; HLA-B-15ZEL allele; HLA-B-27KSH allele; HLA-B-67LAV allele; This submission reports an HLA-B-6701 variant (HLA-B-67LAV) that differs from the published B-6701 sequence, GenBank Accession Number L17005; HLA-B18; HLA-B27 peptide; HLA-B39; HLA-B41 variant; HLA-B61; HLA-Bw-39-DBU allele; HLA-C; HLA-C antigen; HLA-Cw; HLA-Cw1 protein; HLA-Cw2 protein; HLA-Cw3 protein; human leukocyte antigen; Human leukocyte antigen A; Human leukocyte antigen B; Human leukocyte antigen C; human leukocyte antigen-C alpha chain; leucocyte antigen A; leukocyte antigen class I-A; leukocyte antigen class I-B; lymphocyte antigen; major histocompatibility antigen HLA-C; major histocompatibility complex class I A; major histocompatibility HLA-Cw2.2 heavy chain; MGC184092; MGC7052; MHC 1; MHC Class 1; MHC class 1 antigen; MHC class I; MHC class I antigen heavy chain HLA-C; MHC class I antigen HLA-A heavy chain; MHC class I antigen HLA-B alpha chain; MHC class I antigen HLA-B heavy chain; MHC class I antigen SHCHA; MHC class I molecule; MHC class I-like alpha chain membrane-bound type; MHC HLA-A cell surface antigen; MHC HLA-A10-alpha-2 chain precursor; MHC HLA-A2.4a chain; MHC HLA-A24 cell surface glycoprotein; MHC HLA-ABC; MHC HLA-B cell surface glycoprotein; MHC HLA-B transmembrane glycoprotein; MHC HLA-B44; MHC HLA-B7 heavy chain precursor; MHC HLA-Bw46; MHC HLA-C-alpha-2 chain precursor; MHC I HLA; MHC1; similar to Homo sapiens MHC HLA-B39 mRNA with GenBank Accession Number L42024.1; subtype B*3508 differs at residue 156 (Leu->Arg) with subtype B*3501; unnamed protein product
Gene Aliases: AS; B-4901; D6S204; HLA-A; HLA-B; HLA-C; HLA-JY3; HLAA; HLAB; HLAC; HLC-C; MHC; PSORS1
UniProt ID: (Human) P04439, (Human) P01889, (Human) P10321
Entrez Gene ID: (Human) 3105, (Human) 3106, (Human) 3107
T cell mediated cytotoxicity
positive regulation of T cell mediated cytotoxicity
adaptive immune response
immune system process
T cell mediated cytotoxicity directed against tumor cell target
antigen processing and presentation of peptide antigen via MHC class I
antigen processing and presentation of endogenous peptide antigen via MHC class Ib
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent
peptide antigen assembly with MHC class I protein complex
positive regulation of T cell cytokine production
immune response
detection of bacterium
antibacterial humoral response
antigen processing and presentation
antigen processing and presentation of endogenous peptide antigen via MHC class I
positive regulation of interferon-gamma production
CD8-positive, alpha-beta T cell activation
protection from natural killer cell mediated cytotoxicity
antigen processing and presentation of exogenous peptide antigen via MHC class I
innate immune response
defense response to Gram-positive bacterium
T cell receptor signaling pathway
positive regulation of CD8-positive, alpha-beta T cell proliferation
positive regulation of memory T cell activation
positive regulation of CD8-positive, alpha-beta T cell activation
signal transduction
positive regulation of natural killer cell mediated cytotoxicity
regulation of T cell anergy
defense response
regulation of interleukin-12 production
regulation of interleukin-6 production
regulation of dendritic cell differentiation
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