Invitrogen

CaMKII alpha/beta Monoclonal Antibody (22B1), FITC

查看其他5个CaMKII alpha/beta抗体

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持
说明书
实验方案
问题与答案
技术支持

产品信息

MA5-45147

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:1,000
-

免疫组化（石蜡） (IHC (P))

1:100
-

免疫组织化学（多聚甲醛固定） (IHC (PFA))

1:5,000
-

免疫细胞化学 (ICC/IF)

1:1,000
-

酶联免疫吸附实验 (ELISA)

Assay-dependent
-

免疫沉淀 (IP)

Assay-dependent
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

22B1

抗原

Synthetic peptide

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱 spectra

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

9.1mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine

内含物

no preservative

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931601

产品详细信息

1 µg/mL was sufficient for detection of 0.2 µg CamKII by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects phosphorylated CaMKII from rat tissues. This antibody is specific for alpha and beta subunits of CaMKII only when they are phosphorylated at Thr-286/287 (in beta ).

靶标信息

Ca2+/Calmodulin-Dependent Protein Kinase II (CaM Kinase II) is a multi-functional calcium and calmodulin-dependent protein kinase that mediates cellular response to a wide variety of intercellular signals (Kennedy, 1998; Schulman and Hanson, 1993). CaM Kinase II has been shown to regulate diverse cellular functions including synaptic plasticity, neurotransmitter synthesis and release, gene expression, ion channel function, carbohydrate metabolism, cytoskeletal function, and Ca2+-homeostasis (Gleason et al., 2003; Soderling, 2000; Hudmon and Schulman, 2002). Phosphorylation of Thr286 on the kinase produces an autonomously active form of CaM Kinase II (Meng et al., 2003; Picciotto et al., 1993). Autophosphorylation of Thr305 inhibits the activity CaM Kinase II. Phosphorylation at this site appears to reduce the association of CaM Kinase II with the PSD and reduces LTP and learning (Elgersma et al., 2002).

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。
引用该产品

生物信息学

蛋白别名: alpha CaM kinase II; alpha-CaMKII; Ca++/calmodulin-dependent protein kinase II beta subunit; Ca2+/calmodulin-dependent protein kinase II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha; calcium/calmodulin-dependent protein kinase (CaM kinase) II beta; calcium/calmodulin-dependent protein kinase II alpha subunit; calcium/calmodulin-dependent protein kinase II alpha-B subunit; calcium/calmodulin-dependent protein kinase II beta; calcium/calmodulin-dependent protein kinase type II alpha chain; calcium/calmodulin-dependent protein kinase type II beta chain; Calcium/calmodulin-dependent protein kinase type II subunit alpha; Calcium/calmodulin-dependent protein kinase type II subunit beta; CaM kinase II alpha subunit; CaM kinase II beta subunit; CaM kinase II subunit alpha; CaM kinase II subunit beta; CaM-kinase II alpha chain; CaM-kinase II beta chain; CaMK II; CaMK-II alpha subunit; caMK-II subunit alpha; caMK-II subunit beta; CaMKIINalpha; proline rich calmodulin-dependent protein kinase

查看更多 收起

基因别名: CAM2; CAMK2; CAMK2A; CAMK2B; Camk2d; CAMKA; CAMKB; CaMKII; Ck2b; KIAA0968; mKIAA0968; PK2CDD; PKCCD; R74975

查看更多 收起

UniProt ID: (Human) Q9UQM7, (Human) Q13554, (Mouse) P11798, (Rat) P11275, (Rat) P08413, (Mouse) P28652

查看更多 收起

Entrez Gene ID: (Human) 815, (Human) 816, (Mouse) 12322, (Rat) 25400, (Rat) 24245, (Mouse) 12323

查看更多 收起

功能
protein serine/threonine kinase activity calmodulin-dependent protein kinase activity Ras guanyl-nucleotide exchange factor activity protein binding calmodulin binding ATP binding kinase activity glutamate receptor binding protein homodimerization activity actin binding protein kinase binding phospholipase binding nucleotide binding protein kinase activity transferase activity transferase activity, transferring phosphorus-containing groups GTPase activating protein binding non-receptor serine/threonine protein kinase
参与通路
G1/S transition of mitotic cell cycle MAPK cascade response to ischemia protein phosphorylation calcium ion transport Wnt signaling pathway, calcium modulating pathway positive regulation of cardiac muscle cell apoptotic process peptidyl-serine phosphorylation angiotensin-activated signaling pathway positive regulation of GTPase activity protein autophosphorylation regulation of neurotransmitter secretion regulation of neuronal synaptic plasticity positive regulation of NF-kappaB transcription factor activity positive regulation of calcium ion transport interferon-gamma-mediated signaling pathway regulation of cellular response to heat regulation of mitochondrial membrane permeability involved in apoptotic process signal transduction positive regulation of neuron projection development regulation of skeletal muscle adaptation positive regulation of phospholipase A2 activity regulation of long-term neuronal synaptic plasticity cell projection morphogenesis regulation of synapse structural plasticity regulation of calcium ion transport regulation of dendritic spine development positive regulation of dendritic spine morphogenesis positive regulation of synapse maturation phosphorylation ionotropic glutamate receptor signaling pathway inhibitory G-protein coupled receptor phosphorylation regulation of synaptic transmission, cholinergic response to cadmium ion neuromuscular process controlling balance long-term synaptic potentiation activation of meiosis involved in egg activation positive regulation of apoptotic signaling pathway nervous system development cell differentiation
guarantee_icon

性能保障

只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符，您即可享受免费退换货服务。*

了解更多
help_icon

随时为您服务

获取常见问题的专家解答，或者直接联系我们的技术专家，咨询应用、设备、常规产品使用问题。

联系技术支持