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Invitrogen
HO-1 Monoclonal Antibody (1F12-A6), FITC
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FIGURE: 1 / 5
HO-1 Antibody (MA5-45153) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with HO-1 monoclonal antibody (Product # MA5-45153) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Microsome. Endoplasmic reticulum. Localizes to the nucleus upon hypoxia. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-HO-1 anti... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45153
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Dog,
Guinea pig,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1F12-A6
Immunogen
Human HO-1 synthetic peptide, amino acids 1-30
Conjugate
FITC
FITC
FITC
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
9.09mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL was sufficient for detection of HO-1 in 10 µg of mixed human cell line lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary.|Detects 32kDa. Does not cross-react with HO-2.
Target Information
Heme oxygenase (HO) is a microsomal enzyme that catalyzes the oxidation of heme to the antioxidant molecules, biliverdin and carbon monoxide. HO consists of two homologous isozymes, an inducible HO-1 and a constitutively expressed HO-2. HO-1 is induced by a wide variety of stimuli including conditions of oxidative stress, inflammatory agents, transforming growth factor beta and heat shock. The increase in expression of HO-1 is thought to be a cellular defense mechanism against oxidative stress since elevated HO could eventually generate more bilirubin, an anti-oxidant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: heat shock protein 32; heme orygenase 1; heme oxygenase (decycling) 1; heme oxygenase (decyclizing) 1; Heme oxygenase 1; hemoxygenase; HO-1; HSP32; P32 protein
Gene Aliases: D8Wsu38e; Hemox; Heox; HEOXG; Hmox; HMOX1; HO; HO-1; HO1; HSP32
UniProt ID: (Pig) P32394, (Bovine) Q5E9F2, (Rat) P06762, (Mouse) P14901
wound healing involved in inflammatory response
heme oxidation
cellular iron ion homeostasis
apoptotic process
response to oxidative stress
erythrocyte homeostasis
regulation of transcription from RNA polymerase II promoter in response to iron
response to nicotine
heme catabolic process
heme metabolic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation by host of viral transcription
positive regulation of smooth muscle cell proliferation
negative regulation of smooth muscle cell proliferation
iron ion homeostasis
cellular response to arsenic-containing substance
cellular response to cadmium ion
negative regulation of extrinsic apoptotic signaling pathway via death domain receptors
negative regulation of viral life cycle
angiogenesis
response to hypoxia
phospholipid metabolic process
small GTPase mediated signal transduction
regulation of blood pressure
cell death
negative regulation of cell proliferation
intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of muscle cell apoptotic process
cellular response to nutrient
negative regulation of mast cell cytokine production
cellular response to heat
intracellular signal transduction
response to hydrogen peroxide
negative regulation of mast cell degranulation
negative regulation of DNA binding
negative regulation of sequence-specific DNA binding transcription factor activity
negative regulation of neuron apoptotic process
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
response to estrogen
positive regulation of angiogenesis
regulation of sequence-specific DNA binding transcription factor activity
protein homooligomerization
cellular response to hypoxia
oxidation-reduction process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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