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          • Primary Antibodies ›
          • HSP40 Antibodies

          Invitrogen

          HSP40 Monoclonal Antibody (1G10.H8), APC

          View all (27) HSP40 antibodies

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          Cite HSP40 Monoclonal Antibody (1G10.H8), APC

          Additional Information:
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          • Antibody Testing Data (1)
          HSP40 Antibody in Western Blot (WB)
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          HSP40 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          HSP40 Antibody (MA5-45186) in WB

          Western Blot analysis of Yeast Cell lysates showing detection of Hsp40 protein. Load: 15 µg. Blocking: 1.5% BSA for 30 minutes at RT. Samples were incubated with Hsp40 monoclonal antibody (Product # MA5-45186) at 1:1,000 for 2 hours at RT, followed by Sheep Anti-Mouse IgG: HRP for 1 hour at RT. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          HSP40 Antibody in Western Blot (WB)

          Product Details

          MA5-45186

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:2,000
          -

          ELISA (ELISA)

          Assay-dependent
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Yeast

          Host/Isotype

          Mouse / IgG1, kappa

          Class

          Monoclonal

          Type

          Antibody

          Clone

          1G10.H8

          Immunogen

          Full length protein yeast HSP40 (YDJ1)

          Conjugate

          APC APC APC
          • FITC
          • PerCP 
          • Request custom conjugation

          Excitation/Emission Max

          651/660 nm View spectra spectra

          Form

          Liquid

          Concentration

          1 mg/mL

          Amount

          100 µg

          Purification

          Protein G

          Storage buffer

          95.64mM phosphate/2.48mM MES with 0.5M EDTA

          Contains

          no preservative

          Storage conditions

          4°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2931640

          Product Specific Information

          0.5 µg/mL of MA5-45186 was sufficient for detection of 50 ng YDJ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Yeast specific product. Does not cross react with Human, Mouse or Rat.

          Target Information

          TCF-9 (transcription factor 9), also known as C2orf3 (chromosome 2 open reading frame 3), GCF (GC-rich sequence DNA-binding factor) or DNABF, is a 781 amino acid nuclear protein that belongs to the GCF family. Widely expressed, GCF binds the GC-rich sequences of beta-Actin, EGFR and calcium-dependent protease (CANP) promoters. GCF contains multiple phosphoserine and phosphothreonine residues, and two GCF isoforms are produced due to alternative splicing events. GCF is considered a candidate for susceptibility to dyslexia (DYX3) as both genes reside in close proximity on human chromosome 2. Chromosome 2 is the second largest human chromosome and consists of 237 million bases, encodes over 1,400 genes and makes up approximately 8% of the human genome.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Heat shock protein; HSP; HSP 40; NDAJB1

          View more View less

          It has to be done as per old AB suggested Products section.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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