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Invitrogen
Rhodopsin Monoclonal Antibody (1D4), APC
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FIGURE: 1 / 2
Rhodopsin Antibody (MA5-45247) in IHC (P)
Immunohistochemistry analysis using backskin. Fixation involved Bouins Fixative and paraffin-embedded. Samples were incubated with Rhodopsin monoclonal antibody (Product # MA5-45247) at 1:100 for 1 hour at RT, followed by FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Dull epidermal staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-45247
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1D4
Immunogen
Bovine Rhodopsin
Conjugate
APC
APC
APC
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
95.46mM phosphate/2.48mM MES, pH 7.4, with 2mM EDTA
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
1 µg/mL of MA5-45247 was sufficient for detection of rhodopsin in 10 µg of rat eye lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.|Detects approximately 40kDa. Recognizes Rhodopsin (native and recombinant forms). No known reactivity to other proteins. Binds specifically to the C-terminal epitope -T-E-T-S-Q-V-A-P-A-(COOH).
Target Information
Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: L opsin; long wavelength sensitive opsin; LWS opsin; MGC138309; MGC138311; OPN 2; opsin 2; opsin 2, rod pigment; Opsin-2; red opsin; Rhodopsin; rod opsin; RP 4; unnamed protein product
Gene Aliases: CSNBAD1; Noerg1; OPN2; Ops; RHO; RP4
Entrez Gene ID: (Human) 6010, (Mouse) 212541
microtubule cytoskeleton organization
protein phosphorylation
G-protein coupled receptor signaling pathway
visual perception
phototransduction
phototransduction, visible light
response to light stimulus
detection of light stimulus
red, far-red light phototransduction
response to light intensity
gene expression
absorption of visible light
rhodopsin mediated signaling pathway
deactivation of rhodopsin mediated signaling
thermotaxis
photoreceptor cell maintenance
sensory perception of light stimulus
detection of temperature stimulus involved in thermoception
retina development in camera-type eye
cellular response to light stimulus
podosome assembly
transducin-mediated opsin signaling pathway
rod bipolar cell differentiation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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