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Invitrogen
TXNDC5 Monoclonal Antibody (2E7/7), FITC
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图: 1 / 1
TXNDC5 Antibody (MA5-45306) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 4% Formaldehyde for 15 min at RT. Samples were incubated with EndoPDI monoclonal antibody (Product # MA5-45306) at 1:100 for 60 min at RT, followed by Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain used was DAPI (blue) nuclear stain at 1:5,000 for 5 min RT. Localization: Nucleus, Endoplasmic Reticulum, Endoplasmic Reticulum Lumen. Magnification: 60X.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
MA5-45306
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG2a
分类
Monoclonal
类型
Antibody
克隆号
2E7/7
抗原
Synthesized peptide - 12 amino acids long (ADGEDGQDPHSK) corresponding to residues 52 - 63 of the EndoPDI protein
偶联物
FITC
FITC
FITC
激发/发射光谱
498/517 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Protein G
保存液
9.1mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine
内含物
no preservative
保存条件
4°C, store in dark
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
1 µg/mL of MA5-45306 was sufficient for detection of EndoPDI in 20 µg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti -mouse IgG:HRP as the secondary antibody.|Detects approximately 48kDa.
靶标信息
This gene encodes a protein-disulfide isomerase. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Two transcript variants encoding different isoforms have been found for this gene.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Endoplasmic reticulum resident protein 46; ER protein 46; FLJ21353; FLJ90810; MGC3178; thiore; Thioredoxin domain-containing protein 5; Thioredoxin-like protein p46
基因别名: TLP46; TXNDC5; UNQ364/PRO700
Entrez Gene ID: (Human) 81567
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